Investigation on the tolerance of Rhodobacter sphaeroides R-26.1 to metal ions (Abstract/Poster in atti di convegno)

Type
Label
  • Investigation on the tolerance of Rhodobacter sphaeroides R-26.1 to metal ions (Abstract/Poster in atti di convegno) (literal)
Anno
  • 2006-01-01T00:00:00+01:00 (literal)
Alternative label
  • F. Pisani, L.R. Ceci, F. DeLeo, R. Gallerani, F. Italiano, A. Agostiano, F. Milano, M. Trotta, A. Buccolieri, L. Giotta. (2006)
    Investigation on the tolerance of Rhodobacter sphaeroides R-26.1 to metal ions
    in 1st Annual National Congress ItPA, “Proteomics: deciphering the phenotype”, Pisa (ita)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • F. Pisani, L.R. Ceci, F. DeLeo, R. Gallerani, F. Italiano, A. Agostiano, F. Milano, M. Trotta, A. Buccolieri, L. Giotta. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
  • The influence of metal ions on the photosynthetic growth of the facultative anaerobic bacterium Rhodobacter sphaeroides strain R-26.1 is presented. This paper describes the effect of Cr3+ ions on lag phase, maximum growth rate, and pigment-protein complexes expression. The overall results proved that the micro organism is highly tolerant to heavy metal exposure. Preliminary data suggest a role of small soluble proteins in the resistance mechanism to Co2+ exposure. To elucidate the effect of Co2+ on the enzymes involved in tolerance response, a proteomic approach has been used. Using bidimensional electrophoresis, reproducible maps of soluble proteins from R. sphaeroides R-26-1, grown with and without Co2+ (5mM), have been produced. Their analysis, carried out using Image Master 2D 5.0 Platinum Software, reveals around 700 independent spots showing significant differences. The comparative analysis of these spots by MS and MS/MS will be useful to identify proteins directly involved in Co2+ tolerance. In the present work the response to chromium, particularly relevant due to the extreme toxicity of this contaminant, is also tested. The involvement of an efficient resistance mechanism to chromate ions is suggested both by the high EC50 values and the lag-phase lengthening induced by this anion at concentrations above 0.5 mM. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Dipartimento di Chimica - Università di Bari, (b)Istituto per i Processi Chimico Fisici (CNR) - Sezione di Bari (c)Dipartimento di Scienza dei Materiali - Università di Lecce. (d)Dipartimento di Biochimica e Biologia Molecolare - Università degli studi di Bari, (e)Istituto di Biomembrane e Bioenergetica (CNR), Sezione di Trani. (literal)
Titolo
  • Investigation on the tolerance of Rhodobacter sphaeroides R-26.1 to metal ions (literal)
Prodotto di
Autore CNR
Insieme di parole chiave

Incoming links:


Prodotto
Autore CNR di
Insieme di parole chiave di
data.CNR.it