Plant Oil Bodies: Novel Carriers to Deliver Lipophilic Molecules (Articolo in rivista)

Type
Label
  • Plant Oil Bodies: Novel Carriers to Deliver Lipophilic Molecules (Articolo in rivista) (literal)
Anno
  • 2011-01-01T00:00:00+01:00 (literal)
Alternative label
  • Bonsegna S., S. Bettini, R. Pagano, A. Zacheo, V. Vergaro, G. Giovinazzo, G. Caminati, S. Leporatti, L. Valli and A. Santino (2011)
    Plant Oil Bodies: Novel Carriers to Deliver Lipophilic Molecules
    in Applied biochemistry and biotechnology
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Bonsegna S., S. Bettini, R. Pagano, A. Zacheo, V. Vergaro, G. Giovinazzo, G. Caminati, S. Leporatti, L. Valli and A. Santino (literal)
Pagina inizio
  • 792 (literal)
Pagina fine
  • 802 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 163 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1. Inst Sci Food Prod CNR Unit Lecce, I-73100 Lecce, Italy (S. Bonsegna, G.Giovinazzo, A.Santino) 2. Univ Salento, Dipartimento Ingn Innovaz, I-73100 Lecce, Italy (S. Bettini, r. Pagano, L. Valli) 3. NNL Nanosci Inst CNR Lecce, IIT Res Unit, I-73100 Lecce, Italy (A. Zacheo, V. Vergaro, S. Leporatti) 4. Univ Florence, Dept Chem, I-50019 Florence, Italy (G. Caminati) 5. Univ Florence, CSGI Consortium, I-50019 Florence, Italy (G. Caminati) (literal)
Titolo
  • Plant Oil Bodies: Novel Carriers to Deliver Lipophilic Molecules (literal)
Abstract
  • Oil bodies (OBs) are specialised organelles ubiquitously detected in plant oil seeds, which serve as lipid storage compartments. OBs consist of a hydrophobic core of triacylglycerol (TAGs), surrounded by a monolayer of phospholipids (PLs) embedded with some specific proteins with a size ranging from 0.5 to 2 mu m. In this work, we report an easy method to reconstitute OBs starting from their constituents and to encapsulate lipophilic molecules, i.e. the fluorescent fluorescein isothiocyanate (FITC) and carboxyfluorescein (CF), into reconstituted OBs. This methods allowed us to produce OBs 4- to 10-fold smaller (50-200 nm) than the native one and to obtain a good recovery (about 40%) of both the fluorescent compounds used in the present work. The properties of reconstituted OBs were investigated by a combination of Brewster angle microscopy, scanning force microscopy, zeta-potential techniques. OBs were stable and formed ordered monolayers when patterned on hydrophobic substrates whereas they showed a higher tendency to aggregate into larger, coalescing OBs when were deposited onto hydrophilic substrates or at the air/water interface. Furthermore, we verified the uptake of FITC-loaded OBs by the MCF-7 breast cancer cell line. Our results indicated that OBs could be envisaged as novel carriers to deliver hydrophobic bioactive compounds (literal)
Prodotto di
Autore CNR
Insieme di parole chiave

Incoming links:


Autore CNR di
Prodotto
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi
Insieme di parole chiave di
data.CNR.it