Recombinant BCG-Rv1767 amount determines, in vivo, antigen-specific T cells location, frequency, and protective outcome (Articolo in rivista)

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  • Recombinant BCG-Rv1767 amount determines, in vivo, antigen-specific T cells location, frequency, and protective outcome (Articolo in rivista) (literal)
Anno
  • 2010-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.micpath.2010.02.003 (literal)
Alternative label
  • Speranza V., Colone A., Cicconi R., Palmieri G., Giovannini D., Grassi M., Mattei M., Sali M., Delogu G., Andreola F., Colizzi V., Mariani F. (2010)
    Recombinant BCG-Rv1767 amount determines, in vivo, antigen-specific T cells location, frequency, and protective outcome
    in Microbial pathogenesis
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Speranza V., Colone A., Cicconi R., Palmieri G., Giovannini D., Grassi M., Mattei M., Sali M., Delogu G., Andreola F., Colizzi V., Mariani F. (literal)
Pagina inizio
  • 150 (literal)
Pagina fine
  • 159 (literal)
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  • Epub 2010 Feb 26 * Viviana Speranza and Alessia Colone contributed equally to the study. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 48 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1 - Institute of Neurobiology and Molecular Medicine, National Research Council, Via del Fosso del Cavaliere 100, 00133 Rome, Italy; 2 - Biology Department, University of Rome “Tor Vergata”, Via della Ricerca Scientifica, 00133 Rome, Italy; 3 - Animal Breeding Department (STA), University of Rome “Tor Vergata”, Via Montpellier 1, 00133 Rome, Italy; 4 - Catholic University of Sacred Heart, Largo A. Gemelli 8, 00168 Rome, Italy. (literal)
Titolo
  • Recombinant BCG-Rv1767 amount determines, in vivo, antigen-specific T cells location, frequency, and protective outcome (literal)
Abstract
  • One possibility to improve the efficacy of BCG vaccine against TB is to create a recombinant BCG (r-BCG), increasing the expression of mycobacterial antigens, to ameliorate the response to BCG. Here we describe a new r-BCG expressing the gene Rv1767, induced by Mycobacterium tuberculosis during its survival in human macrophages. The r-BCG elicited a specific T cells response in Balb/c mice higher than wt BCG. The r-BCG amount used to immunise mice determined diverse Th1/Th2 equilibriums, which was not the same in spleen and Lymph Nodes. Differences in cytokines production were found for IL-10, IL-4, TNF-alpha, and Arginase-1, which, in some conditions, resulted higher in r-BCG as compared to wt BCG-immunised mice. The immunisation with r-BCG-Rv1767 induced a lesser protective activity than wt BCG in a mouse model of TB. This reduction might likely be explained by the specific T cells phenotype and setting existing before MTB challenge, induced by either the single or the triple dose of r-BCG. The use of this model may help to highlight the capacity of different M. tuberculosis antigens to induce a protective immune response, actually not necessarily embodied by an increased frequency of Antigen-specific effector memory T cells. (literal)
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