Cryopreservation of Fraxinus excelsior L. embryogenic callus by one-step freezing and slow cooling techniques (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Cryopreservation of Fraxinus excelsior L. embryogenic callus by one-step freezing and slow cooling techniques (Articolo in rivista) (literal)

Anno

• 2010-01-01T00:00:00+01:00 (literal)

Alternative label

• E.A. Ozudogru ; M. Capuana ; E. Kaya ; B. Panis ; M. Lambardi (2010)
  Cryopreservation of Fraxinus excelsior L. embryogenic callus by one-step freezing and slow cooling techniques
  in Cryo-letters
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• E.A. Ozudogru ; M. Capuana ; E. Kaya ; B. Panis ; M. Lambardi (literal)

Pagina inizio

• 63 (literal)

Pagina fine

• 75 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://www.cryoletters.org (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 31 (literal)

Rivista

• Cryo-letters (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 13 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 1 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• GYTE / Gebze Yuksek Teknoloji Enstitusu, Istanbul Caddesi, No 101, 41400, Gebze ; IGV / Istituto di Genetica Vegetale, CNR, Via Madonna del Piano 10, 50019, Sesto Fiorentino, Florence, Italy ; GYTE / Gebze Yuksek Teknoloji Enstitusu, Istanbul Caddesi, No 101, 41400, Gebze ; Laboratory of Tropical Crop Improvement, K.U. Leuven, Kasteelpark Arenberg 13, 3001, Leuven, Belgium ; IVALSA / Istituto per la Valorizzazione del Legno e delle Specie Arboree, CNR, Via Madonna del Piano 10, 50019, Sesto Fiorentino, Florence, Italy (literal)

Titolo

• Cryopreservation of Fraxinus excelsior L. embryogenic callus by one-step freezing and slow cooling techniques (literal)

Abstract

• An efficient cryopreservation protocol for the safe storage of Fraxinus excelsior L. embryogenic callus cultures is reported. The cryopreservation methods tested included one-step freezing by means of (i) encapsulation-vitrification; or (ii) encapsulation-dehydration; and (iii) slow cooling using the Nalgene Freezing container, Mr Frosty (R), which produces a temperature decrease of about 1 degrees C min(-1) when placed in a -70 degrees C freezer. None of the one-step freezing techniques was effective for cryopreservation of encapsulated callus masses, irrespective of the cryoprotective treatment applied, i.e., treatment with the PVS2 vitrification solution or physical dehydration with silica gel before direct immersion in liquid nitrogen. On the contrary, when a slow cooling protocol was applied to embryogenic callus which had been pretreated for 60 min with a 210 g l(-1) (0.61 M) sucrose-7.5% DMSO cryoprotective solution, up to about 1.3 g per Petri dish of proliferating callus was observed 42 days after recovery from liquid nitrogen, and cultures were able to produce somatic embryos 8 weeks after transfer to semi-solid medium. TTC staining of callus cultures provided a fast evaluation of culture viability. (literal)

Prodotto di

• Salvaguardia e valorizzazione delle specie arboree LAMBARDI (AG.P04.036.001) (Modulo)
• Istituto di Bioscienze e Biorisorse (IBBR) (Istituto)

Autore CNR

• MAURIZIO LAMBARDI (Persona)
• MAURIZIO CAPUANA (Unità di personale interno)
• ELIF AYLIN OZUDOGRU (Unità di personale esterno)

Insieme di parole chiave

• Parole chiave di "Cryopreservation of Fraxinus excelsior L. embryogenic callus by one-step freezing and slow cooling techniques" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• MAURIZIO LAMBARDI (Persona)
• ELIF AYLIN OZUDOGRU (Unità di personale esterno)
• MAURIZIO CAPUANA (Unità di personale interno)

Prodotto

• Istituto di Bioscienze e Biorisorse (IBBR) (Istituto)
• Salvaguardia e valorizzazione delle specie arboree LAMBARDI (AG.P04.036.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Cryo-letters (Rivista)

Insieme di parole chiave di

• Parole chiave di "Cryopreservation of Fraxinus excelsior L. embryogenic callus by one-step freezing and slow cooling techniques" (Insieme di parole chiave)