Cell cycle and morphological alterations as indicative of apoptosis promoted by UV irradiation in S. Cerevisiae (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Cell cycle and morphological alterations as indicative of apoptosis promoted by UV irradiation in S. Cerevisiae (Articolo in rivista) (literal)

Anno

• 2002-01-01T00:00:00+01:00 (literal)

Alternative label

• Del Carratore R (a), Della Croce C (b), Simili M (a), Taccini E (d), Scavuzzo M (c), Sbrana S. (c) (2002)
  Cell cycle and morphological alterations as indicative of apoptosis promoted by UV irradiation in S. Cerevisiae
  
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Del Carratore R (a), Della Croce C (b), Simili M (a), Taccini E (d), Scavuzzo M (c), Sbrana S. (c) (literal)

Pagina inizio

• 183 (literal)

Pagina fine

• 191 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 513 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto

• questo lavoro, riporta per la prima volta l’induzione di apoptosi indotta da agenti chimici e fisici in lievito. L’importanza del lavoro è data dalla possibilità di usare ceppi di lievito modificati in geni specifici del ciclo cellulare o overesprimenti geni eterologhi per studiare meccanismi evolutivamente conservati del processo di apoptosi. (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Institute of Clinical Physiology, CNR, Cellular Biology and Cytogenetics Unit, Pisa (Italy). (b) Istitute of Mutagenesis and Differenziation, National Council of Research, Via Svezia 10, 56124 Pisa, Italy c Department of Allergy and Clinical Immunology, University Via S. Maria, Pisa, Italy d Department of Animal Pathology, Veterinary Medicine University, Viale delle Piagge, 2 Pisa, Italy (literal)

Titolo

• Cell cycle and morphological alterations as indicative of apoptosis promoted by UV irradiation in S. Cerevisiae (literal)

Abstract

• An apoptotic phenotype induced by oxygen radicals or Bax expression has been observed in Saccharomyces cerevisiae yeast cells by electron and fluorescence microscopy. In this work, we analyzed DNA content and cellular morphology of S. cerevisiae after H(2)O(2) or UV treatment by TdT-mediated dUTP nick end labeling (TUNEL)-test and flow cytofluorimetry. A TUNEL-positive phenotype was observed in both cases, on the same samples a dose-dependent increase in the sub-G(1) population was pointed out by flow cytometry. Sub-G(1) cells were isolated by flow sorting and analyzed by electron microscopy. This population showed condensed chromatin in the nucleus and cell shrinking. This paper reports the first evidence of apoptosis in yeast cells induced by DNA damage after UV irradiation. (literal)

Prodotto di

• Istituto di fisiologia clinica (IFC) (Istituto)

Autore CNR

• MARCELLA SIMILI (Persona)
• MARIA RENATA DEL CARRATORE (Persona)

Insieme di parole chiave

• Parole chiave di "Cell cycle and morphological alterations as indicative of apoptosis promoted by UV irradiation in S. Cerevisiae" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• MARCELLA SIMILI (Persona)
• MARIA RENATA DEL CARRATORE (Persona)

Prodotto

• Istituto di fisiologia clinica (IFC) (Istituto)

Insieme di parole chiave di

• Parole chiave di "Cell cycle and morphological alterations as indicative of apoptosis promoted by UV irradiation in S. Cerevisiae" (Insieme di parole chiave)