Controlled loading of oligodeoxyribonucleotide monolayers onto unoxidized crystalline silicon; fluorescence-based determination of the surface coverage and of the hybridization efficiency; parallel imaging of the process by Atomic Force Microscopy (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Controlled loading of oligodeoxyribonucleotide monolayers onto unoxidized crystalline silicon; fluorescence-based determination of the surface coverage and of the hybridization efficiency; parallel imaging of the process by Atomic Force Microscopy (Articolo in rivista) (literal)

Anno

• 2006-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1093/nar/gnj034 (literal)

Alternative label

• Cattaruzza F. 1; Cricenti A. 1; Flamini A. 1; Girasole M. 1; Longo G. 1; Prosperi T. 1; Andreano G. 2; Cellai L. 2; Chirivino E. 2 (2006)
  Controlled loading of oligodeoxyribonucleotide monolayers onto unoxidized crystalline silicon; fluorescence-based determination of the surface coverage and of the hybridization efficiency; parallel imaging of the process by Atomic Force Microscopy
  in Nucleic acids research
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Cattaruzza F. 1; Cricenti A. 1; Flamini A. 1; Girasole M. 1; Longo G. 1; Prosperi T. 1; Andreano G. 2; Cellai L. 2; Chirivino E. 2 (literal)

Pagina inizio

• 1 (literal)

Pagina fine

• 13 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 34 (literal)

Rivista

• Nucleic acids research (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

• http://dx.doi.org/10.1016/j.tetasy.2006.04.016 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 4 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1 - ISM-CNR, Roma 2 - IC-CNR, Roma (literal)

Titolo

• Controlled loading of oligodeoxyribonucleotide monolayers onto unoxidized crystalline silicon; fluorescence-based determination of the surface coverage and of the hybridization efficiency; parallel imaging of the process by Atomic Force Microscopy (literal)

Abstract

• Unoxidized crystalline silicon, characterized by high purity, high homogeneity, sturdiness and an atomically flat surface, offers many advantages for the construction of electronic miniaturized biosensor arrays upon attachment of biomolecules (DNA, proteins or small organic compounds). This allows to study the incidence of molecular interactions through the simultaneous analysis, within a single experiment, of a number of samples containing small quantities of potential targets, in the presence of thousands of variables. A simple, accurate and robust methodology was established and is here presented, for the assembling of DNA sensors on the unoxidized, crystalline Si(100) surface, by loading controlled amounts of a monolayer DNA-probe through a two-step procedure. At first a monolayer of a spacer molecule, such as 10-undecynoic acid, was deposited, under optimized conditions, via controlled cathodic electrografting, then a synthetic DNA-probe was anchored to it, through amidation in aqueous solution. The surface coverage of several DNA-probes and the control of their efficiency in recognizing a complementary target-DNA upon hybridization were evaluated by fluorescence measurements. The whole process was also monitored in parallel by Atomic Force Microscopy (AFM). (literal)

Prodotto di

• Institute of Crystallography (IC) (Istituto)
• Studi strutturali e funzionali di acidi nucleici (PM.P06.001.002) (Modulo)
• Institute of structure of matter (ISM) (Istituto)
• Microscopia a Scansione a Sonda Locale su sistemi nanostrutturati e materiali biologici (MD.P06.006.001) (Modulo)

Autore CNR

• GIUSEPPINA ANDREANO (Unità di personale esterno)
• TOMMASO PROSPERI (Unità di personale interno)
• ANTONIO CRICENTI (Unità di personale interno)
• ALBERTO FLAMINI (Persona)
• LUCIANO CELLAI (Persona)
• MARCO GIRASOLE (Unità di personale interno)
• FABRIZIO CATTARUZZA (Persona)

Incoming links:

Autore CNR di

• ANTONIO CRICENTI (Unità di personale interno)
• LUCIANO CELLAI (Persona)
• ALBERTO FLAMINI (Persona)
• TOMMASO PROSPERI (Unità di personale interno)
• MARCO GIRASOLE (Unità di personale interno)
• FABRIZIO CATTARUZZA (Persona)
• GIUSEPPINA ANDREANO (Unità di personale esterno)

Prodotto

• Institute of Crystallography (IC) (Istituto)
• Institute of structure of matter (ISM) (Istituto)
• Microscopia a Scansione a Sonda Locale su sistemi nanostrutturati e materiali biologici (MD.P06.006.001) (Modulo)
• Studi strutturali e funzionali di acidi nucleici (PM.P06.001.002) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Nucleic acids research (Rivista)