Chlorophyll fluorescence for evaluating biocide treatments against phototrophic biodetriogens (Articolo in rivista)

Type
Label
  • Chlorophyll fluorescence for evaluating biocide treatments against phototrophic biodetriogens (Articolo in rivista) (literal)
Anno
  • 2002-01-01T00:00:00+01:00 (literal)
Alternative label
  • Tomaselli L., Lamenti G. , Tiano P. (2002)
    Chlorophyll fluorescence for evaluating biocide treatments against phototrophic biodetriogens
    in Annals of microbiology
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Tomaselli L., Lamenti G. , Tiano P. (literal)
Pagina inizio
  • 197 (literal)
Pagina fine
  • 206 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
  • Il sistema messo a punto oltre a permettere il controllo dell'efficacia dei trattamenti biocidi, consente un loro accurato dosaggio individuando rapidamente le concentrazioni necessarie e riducendo in tal modo una loro eccessiva dispersione nell'ambiente (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 52 (literal)
Rivista
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  • 20229 Tomaselli L. in pensione nel corso del 2003 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
  • Sviluppo di un test microalgale basato sulla fluorescenza della clorofilla, quale strumento rapido, sicuro, semplice e non distruttivo per il controllo dell'efficacia dei trattamenti biocidi, negli interventi di restauro di beni culturali. (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Tomaselli L.: CNR Istituto per lo Studio degli Ecosistemi, sede di Firenze Lamenti G.: CNR Istituto per lo Studio degli Ecosistemi, sede di Firenze Tiano P: CNR-ICVBC (literal)
Titolo
  • Chlorophyll fluorescence for evaluating biocide treatments against phototrophic biodetriogens (literal)
Abstract
  • To optimise cleaning procedures and conservation strategies for stone monuments exposed to microbial colonisation, we developed a microalgal test system based on chlorophyll a fluorescence analysis to evaluate the effectiveness of biocide treatments. In comparison with conventional methods, the proposed system is very fast, safe and easy to apply. The system has been tested using as target samples cultures of strains isolated from stone monuments, artificial biofilms developed on small Marble slabs, as well as natural biofilms occurring on marble statues and in which the predominant microorganisms were the phototrophic ones. The samples were treated with different amounts of two biocide products widely used in stone monument restoration treatments, Neo Desogen and Metatin N58-10/101. As reference we used the herbicide Diuron (DCMU). To evaluate the biocide effectiveness we measured the two fluorescence parameters Fv/Fm (maximal quantum yield) and .F/Fm’ (effective quantum yield of photosystem II). We observed a progressive reduction of Fv/Fm and of .F/Fm’ up to the complete inhibition of the latter with the increase of biocide concentrations. This allowed a punctual detection of the biocide concentration effective against the phototrophic biodeteriogens. Once the complete inhibition of .F/Fm’ was obtained, none of the tested samples showed to recover. Data obtained from artificial and natural biofilms were in agreement with those obtained on the cultures of the isolated strains. The results indicate that the microalgal test system based on chlorophyll a fluorescence analysis is a powerful tool for a rapid and not-destructive control of the biocide effectiveness during stone monument cleaning procedures and restoration treatments. (literal)
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