http://www.cnr.it/ontology/cnr/individuo/prodotto/ID170416

Genomic characterization and chromosomal mapping of 5 river buffalo skeletal muscle differentiation master genes (Articolo in rivista)

Type

Articolo in rivista (Classe)
Prodotto della ricerca (Classe)

Label

Genomic characterization and chromosomal mapping of 5 river buffalo skeletal muscle differentiation master genes (Articolo in rivista) (literal)

Anno

2010-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1159/000308359 (literal)

Alternative label

Strazzullo M.; Rossetti C.; Fusco G.; Campanile C.; Vecchio D.; Campanile G.; Perucatti A.; Di Meo G.P.; Filippini F.; Eggen A.; Ferrara L.; D'Esposito M. (2010)
Genomic characterization and chromosomal mapping of 5 river buffalo skeletal muscle differentiation master genes
in Cytogenetic and genome research (Print. ed.); Karger, Basel (Swaziland)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Strazzullo M.; Rossetti C.; Fusco G.; Campanile C.; Vecchio D.; Campanile G.; Perucatti A.; Di Meo G.P.; Filippini F.; Eggen A.; Ferrara L.; D'Esposito M. (literal)

Pagina inizio

221 (literal)

Pagina fine

227 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

128 (literal)

Rivista

Cytogenetic and genome research (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

PMID:20453476 [Epub ahead of print] (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

4 (literal)

Note

ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

National Research Council (CNR), ISPAAM, b CNR, Institute of Genetics and Biophysics A. Buzzati Traverso, and Faculty of Veterinary Medicine, DISCIZIA, Naples , Italy; d Department of Genetics, INRA, Jouy-en-Josas , France; Molecular Biology and Bioinformatics Team MOLBINFO, Department of Biology, University of Padua, Padova , and Institute for Applied Mathematics Mauro Picone, CNR, Naples , Italy (literal)

Titolo

Genomic characterization and chromosomal mapping of 5 river buffalo skeletal muscle differentiation master genes (literal)

Abstract

River buffalo ( Bubalus bubalis , 2n = 50, BBU) is a species of economic relevance in a number of countries. This species shows a very peculiar biology and a great capacity for environmental adaptation. There has been an increasing economic interest as well as a growing demand for a more detailed knowledge of molecular features in this species. From this perspective we report a genomic, transcriptional and cytogenetic analysis of 5 master genes involved in skeletal muscle development. Of these 5 genes, MYOD1 , MYF5 , MYF6 and MYOG belong to the basic helix-loop helix protein family while MSTN belongs to the TNF-B protein family. In mammals, these genes are involved in the early stages of skeletal muscle differentiation, development and regeneration. These pivotal biological functions are finely regulated in a tissue- and temporal-specific manner. We used a comparative genomic approach to obtain the buffalo specific sequences of MYOD1 and MYF6 . The nucleotide sequence similarity and the protein domain conservation of the newly obtained sequences are analysed with respect to bovine and other mammalian species showing sequence similarity. The presence of a polymorphism in MYOD1 coding sequence is described and its possible effect discussed. Using a quantitative PCR approach, we compared the level of the 5 transcripts in adult and fetal muscle. These genes were physically localised on river buffalo R-banded chromosomes by FISH using bovine genomic BAC-clones. Here, we present a genomic and cytogenetic analysis which could offer a background to better characterise the buffalo genes involved in muscle function and which may be responsible for buffalospecific meat features. (literal)

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