Glucose biosensors as models for the development of advanced protein-based biosensors (Articolo in rivista)

Type
Label
  • Glucose biosensors as models for the development of advanced protein-based biosensors (Articolo in rivista) (literal)
Anno
  • 2005-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1039/b513385h (literal)
Alternative label
  • Staiano M.; Bazzicalupo P.; Rossi M.; D'Auria S. (2005)
    Glucose biosensors as models for the development of advanced protein-based biosensors
    in Molecular bioSystems (Print); ROYAL SOC CHEMISTRY, THOMAS GRAHAM HOUSE,, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND (Regno Unito)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Staiano M.; Bazzicalupo P.; Rossi M.; D'Auria S. (literal)
Pagina inizio
  • 354 (literal)
Pagina fine
  • 362 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 1 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 5-6 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Institute of Protein Biochemistry, CNR, Italian National Research Council, Via Pietro Castellino, 111 80131 Naples, Italy. Institute of Genetics and Biophysics, CNR, Naples, Italy (literal)
Titolo
  • Glucose biosensors as models for the development of advanced protein-based biosensors (literal)
Abstract
  • Glucose sensing is used as a model to explore the advantages and problems deriving from the use of either enzymes or sugar binding proteins to develop stable fluorescence biosensors. We report on a novel approach to address the problem of substrate consumption by sensors based on enzymes, namely the utilization of apo-enzymes as non-active forms of the protein which are still able to bind the substrate/ligand. We also review studies in which derivatization of a naturally thermostable sugar-binding protein with a fluorescent probe allows quantitative monitoring of glucose binding even after immobilization on a solid support. (literal)
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