http://www.cnr.it/ontology/cnr/individuo/prodotto/ID170316

Breath figures-mediated microprinting allows for versatile applications in molecular biology (Articolo in rivista)

Type

Label

Breath figures-mediated microprinting allows for versatile applications in molecular biology (Articolo in rivista) (literal)

Anno

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

Alternative label

Galeotti F.; Chiusa I.; Morello L.; Gianì S.; Breviario D.; Hatz S.; Damin F.; Chiari M.; Bolognesi A. (2009)
Breath figures-mediated microprinting allows for versatile applications in molecular biology
in European Polymer Journal; Editore: Elsevier, Oxford (Regno Unito)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Galeotti F.; Chiusa I.; Morello L.; Gianì S.; Breviario D.; Hatz S.; Damin F.; Chiari M.; Bolognesi A. (literal)

Pagina inizio

Pagina fine

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

Si tratta di un lavoro multidisciplinare, frutto della collaborazione tra gruppi appartenenti a tre Istituti CNR milanesi, in cui competenze nel settore chimico e in quello biologico sono state integrate per lo sviluppo di applicazioni innovative nel campo dei materiali polimerici microstrutturati. (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

Rivista

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto

Pubblicazione scientifica riguardante lapplicazione di particolari tecniche di sintesi dei polimeri per la produzione di stampi adatti al trasferimento di molecole biologiche su substrati sulle quali possono essere immobilizzate a analizzate. (literal)

Note

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

CI, ML, GS, BD - IBBA-CNR, Milano; GF,HS, BA - ISMAC-CNR, Milano e Polo Biotecnologico, Via Fantoli, Milano; DF, CM - ICRM-CNR, Milano (literal)

Titolo

Breath figures-mediated microprinting allows for versatile applications in molecular biology (literal)

Abstract

Softlithographic processes are currently applied in different fields ranging from microelectronics to biology. One of the main advantages of these techniques stands in the low cost and in its easy use without the need of expensive apparatus. The available procedure is simple and particularly suitable for biological tests: a biomolecule is printed on a suitable substrate and its presence can be further recognized with a given reactants. The use of polydimethylsiloxane (PDMS) is a key step in this technology because printing from PDMS mould is an easy process. We have recently shown that the hexagonal ordered pattern which is obtained by breath figure method on a polystyrene film, generated by arrangements of micrometric holes on the film surface, can be reproduced by PDMS obtaining a positive mould, formed by regularly arranged micrometric protuberances. This mould can be \"inked\" with a suitable biomolecule solution which is subsequently printed on the desired substrate. With this method no photolithographic step is necessary; regular spots of a few microns can be easily produced without the need of sophisticated apparatus. We report some examples of printing with different molecules which are then recognized by specific reactants. A further advantage of the method we present in this work is related to the multiple printing of the same labelled molecule with the same mould onto several substrates without significant loss of signal. (literal)

Editore