Vaccination with Filamentous Bacteriophages trageting DEC-205 induces DC maturation and potent anti-tumor T cell response in absence of adjuvants (Articolo in rivista)

Type
Label
  • Vaccination with Filamentous Bacteriophages trageting DEC-205 induces DC maturation and potent anti-tumor T cell response in absence of adjuvants (Articolo in rivista) (literal)
Anno
  • 2011-01-01T00:00:00+01:00 (literal)
Alternative label
  • Sartorius R, Bettua C, D'Apice L, Caivano A, Trovato M, Russo D, Zanoni I, Granucci F, Mascolo D, Barba P, Del Pozzo G, De Berardinis P. (2011)
    Vaccination with Filamentous Bacteriophages trageting DEC-205 induces DC maturation and potent anti-tumor T cell response in absence of adjuvants
    in European Journal of Immunology
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Sartorius R, Bettua C, D'Apice L, Caivano A, Trovato M, Russo D, Zanoni I, Granucci F, Mascolo D, Barba P, Del Pozzo G, De Berardinis P. (literal)
Pagina inizio
  • 2573 (literal)
Pagina fine
  • 2584 (literal)
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  • 41 (literal)
Rivista
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  • PMID: 21688262 (literal)
Note
  • ISI Web of Science (WOS) (literal)
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  • CNR-IGB\"ABT\" (literal)
Titolo
  • Vaccination with Filamentous Bacteriophages trageting DEC-205 induces DC maturation and potent anti-tumor T cell response in absence of adjuvants (literal)
Abstract
  • The efficacy of a new vaccine-delivery vector, based on the filamentous bacteriophage fd displaying a single-chain antibody fragment known to bind the mouse DC surface molecule DEC-205, is reported. We demonstrate both in vitro and in vivo an enhanced receptor-mediated uptake of phage particles expressing the anti-DEC-205 fragment by DCs. We also report that DCs targeted by fd virions in the absence of other stimuli produce IFN-± and IL-6, and acquire a mature phenotype. Moreover, DC-targeting with fd particles double-displaying the anti-DEC-205 fragment on the pIII protein and the OVA(257-264) antigenic determinant on the pVIII protein induced potent inhibition of the growth of the B16-OVA tumor in vivo. This protection was much stronger than other immunization strategies and similar to that induced by adoptively transferred DCs. Since targeting DEC-205 in the absence of DC activation/maturation agents has previously been described to result in tolerance, the ability of fd bacteriophages to induce a strong tumor-specific immune response by targeting DCs through DEC-205 is unexpected, and further validates the potential employment of this safe, versatile and inexpensive delivery system for vaccine formulation (literal)
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