http://www.cnr.it/ontology/cnr/individuo/prodotto/ID169954
The photosynthetic membrane proteome of Rhodobacter sphaeroides R-26.1 exposed to cobalt (Articolo in rivista)
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- The photosynthetic membrane proteome of Rhodobacter sphaeroides R-26.1 exposed to cobalt (Articolo in rivista) (literal)
- Anno
- 2011-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1016/j.resmic.2011.04.008 (literal)
- Alternative label
Italiano F, D'Amici GM, Rinalducci S, De Leo F, Zolla L, Gallerani R, Trotta M, Ceci LR. (2011)
The photosynthetic membrane proteome of Rhodobacter sphaeroides R-26.1 exposed to cobalt
in Research in microbiology (Paris)
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- Italiano F, D'Amici GM, Rinalducci S, De Leo F, Zolla L, Gallerani R, Trotta M, Ceci LR. (literal)
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- Italian National Research Council (CNR), Institute for Chemical-Physical Processes, Via Orabona 4, 70124 Bari, Italy
University of Tuscia, Department of Environmental Sciences, Largo dell'Università, 01100 Viterbo, Italy
CNR, Institute for Biomembranes and Bioenergetics, Via Amendola 165/A, 70126 Bari, Italy
University of Bari, Department of Biochemistry and Molecular Biology, Via Amendola 165/A, 70126 Bari, Italy (literal)
- Titolo
- The photosynthetic membrane proteome of Rhodobacter sphaeroides R-26.1 exposed to cobalt (literal)
- Abstract
- Cells of the carotenoidless strain R-26.1 of Rhodobacter sphaeroides were grown in the presence of a high concentration (5 mM) of cobalt ions. The photosynthetic intracytoplasmic membranes were isolated and investigated by proteomic analysis using non-denaturating blue native electrophoresis in combination with LC-ESI-MS/MS. Comparison with intracytoplasmic membranes of cells grown under control conditions showed a change in the relative amount of proteins belonging to the photosynthetic apparatus, with net downregulation of light-harvesting complexes and increased concentration of the nude reaction center (RC), as well as upregulation of enzymes related to chemoorganotrophy. These effects represent possible bacterial adaptation so as to retrieve energy for metabolic processes from sources alternative to less efficient photosynthesis. The influence of cobalt on the photochemistry of the RC in cell extracts was also investigated by charge recombination. The kinetics of the charge recombination reaction was found to be slower in extracts from cells exposed to Co(2+), indicating that the reorganization of the photosynthetic apparatus also involves its photochemical core. (literal)
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