http://www.cnr.it/ontology/cnr/individuo/prodotto/ID169231
Structural analysis of BldR from Sulfolobus solfataricus provides insights into the molecular basis of transcriptional activation in Archaea by MarR family proteins (Articolo in rivista)
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- Structural analysis of BldR from Sulfolobus solfataricus provides insights into the molecular basis of transcriptional activation in Archaea by MarR family proteins (Articolo in rivista) (literal)
- Anno
- 2009-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1016/j.jmb.2009.03.030 (literal)
- Alternative label
Di Fiore A.; Fiorentino G.; Vitale R.M.; Ronca R.; Amodeo P.; Pedone C.; Bartolucci S.; De Simone G. (2009)
Structural analysis of BldR from Sulfolobus solfataricus provides insights into the molecular basis of transcriptional activation in Archaea by MarR family proteins
in Journal of Molecular Biology; Academic Press Ltd., Elsevier Science Ltd., London (Regno Unito)
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Di Fiore A.; Fiorentino G.; Vitale R.M.; Ronca R.; Amodeo P.; Pedone C.; Bartolucci S.; De Simone G. (literal)
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- 1Istituto di Biostrutture e
Bioimmagini-CNR, via
Mezzocannone 16,
80134 Naples, Italy
2Dipartimento di Biologia
Strutturale e Funzionale,
Università degli Studi di Napoli
Federico II, Complesso
Universitario Monte S. Angelo,
via Cinthia, 80126 Naples, Italy
3Istituto di Chimica e
Biomolecolare-CNR, via Campi
Flegrei 34, 80078 Pozzuoli, Italy (literal)
- Titolo
- Structural analysis of BldR from Sulfolobus solfataricus provides insights into the molecular basis of transcriptional activation in Archaea by MarR family proteins (literal)
- Abstract
- The multiple antibiotic resistance regulator (MarR) family constitutes a
significant class of transcriptional regulators whose members control a
variety of important biological functions such as regulation of response to
environmental stress, control of virulence factor production, resistance to
antimicrobial agents, and regulation of aromatic catabolic pathways.
Although the majority of MarR family members have been characterized
as transcriptional repressors, a few examples of transcriptional activators
have also been reported. BldR is a newly identified member of this family
that has been demonstrated to act as a transcriptional activator in stress
response to aromatic compounds in the crenarchaeon Sulfolobus solfataricus.
In this work, we report findings on the BldR X-ray crystal structure
and present a molecular modeling study on the complex that this protein
forms with its cognate DNA sequence, thus providing the first detailed
description of the DNA-binding mechanism of an archaeal activator
belonging to the MarR family. Two residues responsible for the high
binding specificity of this transcriptional regulator were also identified.
Our studies demonstrated that, in Archaea, the capability of MarR family
members to act as activators or repressors is not related to a particular
DNA-binding mechanism but rather could be due to the position of the
binding site on the target DNA. Moreover, since genes encoding MarR
proteins often control transcription of operons that encode for multisubstrate
efflux pumps, our results also provided important insights for
the identification of new tools to overcome the microorganism's multidrug
resistance (literal)
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