http://www.cnr.it/ontology/cnr/individuo/prodotto/ID169047

Nanostructured silver-based surfaces: new emergent methodologies for an easy detection of analytes (Articolo in rivista)

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Nanostructured silver-based surfaces: new emergent methodologies for an easy detection of analytes (Articolo in rivista) (literal)

Anno

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Alternative label

Staiano M.; Matveeva E.G.; Rossi M.; Crescenzo R.; Gryczynski Z.; Gryczynski I.; Iozzino L.; Akopova I.; D'Auria S. (2009)
Nanostructured silver-based surfaces: new emergent methodologies for an easy detection of analytes
in ACS applied materials & interfaces (Print); ACS, American chemical society, Washington, DC (Stati Uniti d'America)
(literal)

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Staiano M.; Matveeva E.G.; Rossi M.; Crescenzo R.; Gryczynski Z.; Gryczynski I.; Iozzino L.; Akopova I.; D'Auria S. (literal)

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Note

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Laboratory for Molecular Sensing, Istituto di Biochimica delle Proteine-Consiglio Nazionale delle Richerche, Naples, Italy, Center for Commercialization of Fluorescence Technologies, Department of Molecular Biology and Immunology, University of North Texas, Health Science Center, Fort Worth, Texas, Institute of Food Sciences, Consiglio Nazionale delle Richerche, Avellino, Italy, and School of Veterinary Medicine, University of Naples Federico II, Naples, Italy (literal)

Titolo

Nanostructured silver-based surfaces: new emergent methodologies for an easy detection of analytes (literal)

Abstract

In this work, we describe how to realize a new sensing platform for an easy and fast detection of analytes. In particular, we utilized enhanced fluorescence emission on silver island films (SIFs) coupled to the total internal reflection fluorescence mode (TIRF) to develop a new assay format for the detection of target analytes. Here, as an example, we report on the detection of the toxic peptides present in gliadin (Gli). Our assay was performed as follows: (1) gliadin was first captured on surfaces coated with anti-Gli antibodies; (2) the surfaces were then incubated with fluorophore-labeled anti-Gli antibodies; (3) the signal from the fluorophore-labeled anti-Gli antibody bound to the antigen was detected by TIRF. The system was examined on glass surfaces and on SIFs. We observed a relevant enhancement of the signal from SIFs compared to the signal from the glass substrate not modified with a SIF. In addition, the estimated detection limit (EDL) of our methodology was 60 ng/mL (or lower). This limit is therefore lower than the clinical cut-off for Gli presence in food for celiac patients. The advantage of our method is a reduced number of testing steps, which allows for easy detection of residual toxic peptides in food labeled as gluten free. The proposed technology can be easily expanded to the determination of different target analytes. (literal)

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