Use of fusion proteins and procaryotic display systems for delivery of HIV-1 antigens: development of novel vaccines for HIV-1 infection. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Use of fusion proteins and procaryotic display systems for delivery of HIV-1 antigens: development of novel vaccines for HIV-1 infection. (Articolo in rivista) (literal)

Anno

• 2003-01-01T00:00:00+01:00 (literal)

Alternative label

• De Berardinis P., Sartorius R.,Caivano A., Mascolo D., Domingo G.J., Del Pozzo G., Gaubin M., Perham R.N., Piatier-Tonneau D., Guardiola J. (2003)
  Use of fusion proteins and procaryotic display systems for delivery of HIV-1 antigens: development of novel vaccines for HIV-1 infection.
  in Current HIV research (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• De Berardinis P., Sartorius R.,Caivano A., Mascolo D., Domingo G.J., Del Pozzo G., Gaubin M., Perham R.N., Piatier-Tonneau D., Guardiola J. (literal)

Pagina inizio

• 441 (literal)

Pagina fine

• 446 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 1 (literal)

Rivista

• Current HIV research (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Titolo

• Use of fusion proteins and procaryotic display systems for delivery of HIV-1 antigens: development of novel vaccines for HIV-1 infection. (literal)

Abstract

• Two non-pathogenic scaffolds (represented by the filamentous bacteriophage fd and the dihydrolipoyl acetyltransferase E2 protein of the Bacillus stearothermophilus pyruvate dehydrogenase (PDH) complex) able to deliver human immunodeficiency virus (HIV)-1 antigenic determinants, were designed in our laboratories and investigated in controlled assay conditions. Based on a modification of the phage display technology, we developed an innovative concept for a safe and inexpensive vaccine in which conserved antigenic determinants of HIV-1 reverse transcriptase (RTase) were inserted into the N-terminal region of the major pVIII coat protein of bacteriophage df virions. Analogously, we developed another antigen delivery system based on the E2 component from the PDH complex and capable of displaying large intact proteins on the surface of an icosahedral lattice. Our data show that both of these systems can deliver B and T epitopes to their respective presentation compartments in target cells and trigger a humoral response as well as a potent helper and cytolytic response in vitro and in vivo. (literal)

Prodotto di

• DR. DE BERARDINIS: SV.P03.006 (SV.P14.003.001) (Modulo)
• Istituto di genetica e biofisica \"Adriano Buzzati Traverso\" (IGB) (Istituto)
• Institute of protein biochemistry (IBP) (Istituto)

Autore CNR

• ANTONELLA CAIVANO (Unità di personale esterno)
• ROSSELLA SARTORIUS (Unità di personale esterno)
• JOHN GUARDIOLA (Persona)
• PIERGIUSEPPE DE BERARDINIS (Unità di personale interno)
• GIOVANNA DEL POZZO (Unità di personale interno)

Incoming links:

Prodotto

• Istituto di genetica e biofisica \"Adriano Buzzati Traverso\" (IGB) (Istituto)
• Institute of protein biochemistry (IBP) (Istituto)
• DR. DE BERARDINIS: SV.P03.006 (SV.P14.003.001) (Modulo)

Autore CNR di

• PIERGIUSEPPE DE BERARDINIS (Unità di personale interno)
• GIOVANNA DEL POZZO (Unità di personale interno)
• ANTONELLA CAIVANO (Unità di personale esterno)
• ROSSELLA SARTORIUS (Unità di personale esterno)
• JOHN GUARDIOLA (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Current HIV research (Rivista)