pH-sensitive fluorescent dye as probe for proton uptake in photosynthetic reaction centers (Articolo in rivista)

Type
Label
  • pH-sensitive fluorescent dye as probe for proton uptake in photosynthetic reaction centers (Articolo in rivista) (literal)
Anno
  • 2004-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.bioelechem.2003.10.015 (literal)
Alternative label
  • Agostiano, A., Mavelli, F., Milano, F., Giotta, L., Trotta, M., Nagy, L. & Maroti, P (2004)
    pH-sensitive fluorescent dye as probe for proton uptake in photosynthetic reaction centers
    in Bioelectrochemistry (Amsterdam)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Agostiano, A., Mavelli, F., Milano, F., Giotta, L., Trotta, M., Nagy, L. & Maroti, P (literal)
Pagina inizio
  • 125 (literal)
Pagina fine
  • 128 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 63 (literal)
Rivista
Note
  • Scopu (literal)
  • PubMe (literal)
  • Google Scholar (literal)
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Istituto per i Processi Chimico-Fisici Sezione di Bari Dipartimento di Chimica Università di Bari Dipartimento di Scienza dei Materiali Università di Lecce Department of Biophysics University of Szeged (literal)
Titolo
  • pH-sensitive fluorescent dye as probe for proton uptake in photosynthetic reaction centers (literal)
Abstract
  • Isolated and purified reaction center from Rhodobacter sphaeroides R-26.1 were solubilised in detergent with excess quinone and external electron donors and illuminated in the presence of pyranine. The pH change accompanying the reaction center photocycle was monitored by recording the variation of the pyranine fluorescence intensity. Using QB-depleted reaction centers or blocking the photocycle with terbutryne strongly reduced the pH-change. The usefulness and limits of this technique in monitoring the pH changes during the RC photocycle are also discussed. (literal)
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