http://www.cnr.it/ontology/cnr/individuo/prodotto/ID168880

Mechanisms for the coupling of cannabinoid receptors to intracellular calcium mobilization in rat insulinoma beta-cells. (Articolo in rivista)

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Mechanisms for the coupling of cannabinoid receptors to intracellular calcium mobilization in rat insulinoma beta-cells. (Articolo in rivista) (literal)

Anno

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Alternative label

De Petrocellis L., Marini P., Matias I., Schiano Moriello A., Starowicz K., Cristino L., Nigam S., Di Marzo V. (2007)
Mechanisms for the coupling of cannabinoid receptors to intracellular calcium mobilization in rat insulinoma beta-cells.
in Experimental cell research; Elsevier Inc., San Diego (Stati Uniti d'America)
(literal)

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De Petrocellis L., Marini P., Matias I., Schiano Moriello A., Starowicz K., Cristino L., Nigam S., Di Marzo V. (literal)

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1. CNR, Inst Biomol Chem, Endocannabinoid Res Grp, I-80078 Naples, Italy 2. Univ Roma La Sapienza, Dept Human Physiol & Pharmacol, Rome, Italy 3. Free Univ Berlin, Univ Klinikum Benjamin Franklin, Eicosanoid & Lipid Res Div, D-1000 Berlin, Germany 4. Free Univ Berlin, Univ Klinikum Benjamin Franklin, Ctr Expt Gynecol & Breast Res, D-1000 Berlin, Germany 5. CNR, Inst Cybernet, Endocannabinoid Res Grp, I-80078 Naples, Italy (literal)

Titolo

Mechanisms for the coupling of cannabinoid receptors to intracellular calcium mobilization in rat insulinoma beta-cells. (literal)

Abstract

In RIN m5F rat insulinoma beta-cells, agonists at cannabinoid CB(1) receptors modulate insulin release. Here we investigated in these cells the effect of the activation of cannabinoid CB(1) and CB(2) receptors on intracellular Ca(2+) ([Ca(2+)](i)). The CB(1) agonist arachidonoyl-chloro-ethanolamide (ACEA), and the CB(2) agonist JWH133, elevated [Ca(2+)](i) in a way sensitive to the inhibitor of phosphoinositide-specific phospholipase C (PI-PLC), U73122 (but not to pertussis toxin and forskolin), and independently from extracellular Ca(2+). PI-PLC-dependent Ca(2+) mobilization by ACEA was entirely accounted for by activation of inositol-1,3,4-phosphate (IP(3)) receptors on the endoplasmic reticulum (ER), whereas the effect of JWH133 was not sensitive to all tested inhibitors of IP(3) and ryanodine receptors. ACEA, but not JWH133, significantly inhibited the effect on [Ca(2+)](i) of bombesin, which acts via G(q/11)- and PI-PLC-coupled receptors in insulinoma cells. The endogenous CB(1) agonists, anandamide and N-arachidonoyldopamine, which also activate transient receptor potential vanilloid type 1 (TRPV1) receptors expressed in RIN m5F cells, elevated [Ca(2+)](i) in the presence of (literal)

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