http://www.cnr.it/ontology/cnr/individuo/prodotto/ID168854

Cell-death mediated antiviral effect of chitosan in Tobacco. (Articolo in rivista)

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Cell-death mediated antiviral effect of chitosan in Tobacco. (Articolo in rivista) (literal)

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Alternative label

IRITI, M., SIRONI, M., GOMARASCA, S., CASAZZA, A.P., SOAVE, C. & FAORO, F. (2006)
Cell-death mediated antiviral effect of chitosan in Tobacco.
in Plant physiology and biochemistry (Paris); Elsevier France, Editions Scientifiques Medicales Elsevier, Paris (Francia)
(literal)

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IRITI, M., SIRONI, M., GOMARASCA, S., CASAZZA, A.P., SOAVE, C. & FAORO, F. (literal)

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IRITI M., SIRONI M., GOMARASCA S., SOAVE C., UNIVERSITA' DEGLI STUDI DI MILANO (literal)

Titolo

Abstract

The antiviral activity induced by chitosan (CHT), and the mechanisms underlying it, were studied in a tobacco-tobacco necrosis necrovirus (TNV) pathosystem. Treatments with 0.1% CHT enhanced tobacco inducible defenses against TNV, reducing significantly the virus-induced necrotic lesions (in a range from 32% to 83%). In planta, this resistance was associated with a network of callose deposits, micro-oxidative bursts and micro-hypersensitive responses (micro-HRs), as assessed, respectively, by aniline blue, 3,3'-diaminobenzidine (DAB) and Evans blue staining. In order to verify if CHT-elicited cell death could be regarded as an apoptotic process, tobacco bright yellow 2 (BY2) cell cultures were treated with different CHT concentrations, ranging from 0.01% to 0.1%. After 6 h about half of the cultured cells incubated in 0.05% CHT were Evans blue positive, showing some typical morphological features of apoptosis, such as cytoplasm shrinkage and nuclear chromatin condensation. The latter was checked by 4',6-diamino-2-phenylindole (DAPI) and ethidium bromide nuclear staining and was visible already at 2 h after treatment. Moreover, the cell death kinetic induced by CHT was delayed by Verapamil(R), a calcium channel blocker. Finally, electrophoresis of genomic DNA extracted from cultured cell after 48 h treatment showed internucleosomal fragmentation, visualized as a distinct ladder of DNA bands corresponding to oligonucleosomal units. (literal)

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