Simultaneous intracellular chloride and pH measurements using a GFP-based sensor (Articolo in rivista)

Type
Label
  • Simultaneous intracellular chloride and pH measurements using a GFP-based sensor (Articolo in rivista) (literal)
Anno
  • 2010-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1038/nmeth.1471 (literal)
Alternative label
  • Arosio D; Ricci F; Marchetti L; Gualdani R; Albertazzi L; Beltram F. (2010)
    Simultaneous intracellular chloride and pH measurements using a GFP-based sensor
    in Nature methods
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Arosio D; Ricci F; Marchetti L; Gualdani R; Albertazzi L; Beltram F. (literal)
Pagina inizio
  • 516 (literal)
Pagina fine
  • 518 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://dx.doi.org/10.1038/nmeth.1471 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 7 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 7 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1. CNR, Natl Enterprise NanoSci & NanoTechnol, Ist Nanosci, I-56100 Pisa, Italy 2. Scuola Normale Super Pisa, Pisa, Italy 3. CNR, Ist Biofis, Trento Povo, Italy 4. Natl Enterprise NanoSci & NanoTechnol, Italian Inst Technol, Ctr Nanotechnol Innovat, Pisa, Italy (literal)
Titolo
  • Simultaneous intracellular chloride and pH measurements using a GFP-based sensor (literal)
Abstract
  • Chloride and protons perform important closely related roles in many cellular responses. Here we developed a ratiometric biosensor, ClopHensor, based on a highly chloride-sensitive Aequorea victoria GFP variant that is suited for the combined real-time optical detection of pH changes and chloride fluxes in live cells. We detected high chloride concentration in large dense-core exocytosis granules by targeting ClopHensor to these intracellular compartments. (literal)
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