http://www.cnr.it/ontology/cnr/individuo/prodotto/ID168819
Simultaneous intracellular chloride and pH measurements using a GFP-based sensor (Articolo in rivista)
- Type
- Label
- Simultaneous intracellular chloride and pH measurements using a GFP-based sensor (Articolo in rivista) (literal)
- Anno
- 2010-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1038/nmeth.1471 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Arosio D; Ricci F; Marchetti L; Gualdani R; Albertazzi L; Beltram F. (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
- http://dx.doi.org/10.1038/nmeth.1471 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- 1. CNR, Natl Enterprise NanoSci & NanoTechnol, Ist Nanosci, I-56100 Pisa, Italy
2. Scuola Normale Super Pisa, Pisa, Italy
3. CNR, Ist Biofis, Trento Povo, Italy
4. Natl Enterprise NanoSci & NanoTechnol, Italian Inst Technol, Ctr Nanotechnol Innovat, Pisa, Italy (literal)
- Titolo
- Simultaneous intracellular chloride and pH measurements using a GFP-based sensor (literal)
- Abstract
- Chloride and protons perform important closely related roles in many cellular responses. Here we developed a ratiometric biosensor, ClopHensor, based on a highly chloride-sensitive Aequorea victoria GFP variant that is suited for the combined real-time optical detection of pH changes and chloride fluxes in live cells. We detected high chloride concentration in large dense-core exocytosis granules by targeting ClopHensor to these intracellular compartments. (literal)
- Prodotto di
- Autore CNR
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