http://www.cnr.it/ontology/cnr/individuo/prodotto/ID168778
A general method for the extraction of citrus leaf proteins and separation by 2D electrophoresis: a follow up. (Articolo in rivista)
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- Label
- A general method for the extraction of citrus leaf proteins and separation by 2D electrophoresis: a follow up. (Articolo in rivista) (literal)
- Anno
- 2007-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1016/j.jchromb.2006.09.025 (literal)
- Alternative label
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- Maserti B.E.; Della Croce C.M.; Luro F.; Morillon R.; Cini M.; Caltavuturo L. (literal)
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- Science direct - Elsevier (literal)
- Scopu (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Luro F, National Institute of Agricultural Research (INRA), Agricultural Research Station (SRA INRA), Corse, France
Morillon R, Co-operation and Research on Agricultural Development (CIRAD), Montpellier, France (literal)
- Titolo
- A general method for the extraction of citrus leaf proteins and separation by 2D electrophoresis: a follow up. (literal)
- Abstract
- With the aim of studying differentially expressed proteins as a function of abiotic and biotic stress in citrus plants, we optimized a protocol for the extraction of total leaf proteins and their 2-DE separation using commercially available immobilized pH gradient strips (IPGs) in the first dimension. Critical factors for good reproducibility of citrus leaf protein separation were identified: trichloroacetic acid (TCA)/acetone precipitation after extraction in lysis buffer, sample fractionation on narrow range overlapping IPGs and sample-cup loading at the anodic or cathodic end of the strip. The use of thiourea and a strong detergent (C7BzO) in the solubilization/rehydration buffer, coupled with the increase to 10% of SDS in the equilibration buffer before the second dimension seemed to affect positively the resolution of basic proteins. Using our protocol we resolved about 30 basic proteins on 6.3-8.3 pH range strips. Further, our protocol was successfully applied reproducibly on the analysis of control and salt exposed leaf samples of Citrus reshni Hort. Ex Tan. (literal)
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