http://www.cnr.it/ontology/cnr/individuo/prodotto/ID168721
Comparative analysis of new innovative vaccine formulations based on the use of procaryotic display systems (Articolo in rivista)
- Type
- Label
- Comparative analysis of new innovative vaccine formulations based on the use of procaryotic display systems (Articolo in rivista) (literal)
- Anno
- 2007-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1016/j.vaccine.2006.11.047 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- D'Apice L.; Sartorius R.; Caivano A.; Mascolo D.; Del Pozzo G.; Scotto Di Mase D.; Ricca E.; Li Pira G.; Manca F.; Malanga D.; De Palma R.; De Berardinis P. (literal)
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- ISI Web of Science (WOS) (literal)
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- Institute of Protein Biochemistry, C.N.R.,Via P. Castellino 111, 80131 Naples, Italy
Institute of Genetics and Biophysics, C.N.R., Via P. Castellino 111, 80131 Naples, Italy
Department of Structural and Functional Biology, University Federico II, Via Cinthia, MSA, 80126 Naples, Italy
Unit of Viral Immunology, Advanced Biotechnology Center, Largo Benzi 10, 16132 Genoa, Italy
Laboratory of Clinical and Experimental Immunology, G. Gaslini Institute, Largo G. Gaslini 5, 16148 Genoa, Italy
Department of Internal Medicine, Second University of Naples, Via S. Pansini 5, 80131 Naples, Italy (literal)
- Titolo
- Comparative analysis of new innovative vaccine formulations based on the use of procaryotic display systems (literal)
- Abstract
- A T helper epitope was expressed in three innovative delivery vehicles recently developed in our laboratories and based respectively, on the filamentous bacteriophage fd, the E2 protein from the PDH complex of Bacillus stearothermophilus and the protein CotC of Bacillus subtilis spores. Studies of antigenicity and immunogenicity were performed by using a specific T cell hybridoma and by priming mononuclear cells isolated from the venous blood of human donors. The results indicate that the E2 system is the best suited for inducing a specific immune response towards a CD4 T cell epitope. Importantly, TCR clonal analysis demonstrated the persistence over years of a previously described antigen specific clonotype and its presence correlates with the immunogenic strength of the antigen delivery system. (literal)
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