Exploiting nanotechnologies and TRPV1 channels to investigate the putative anandamide membrane transporter. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Exploiting nanotechnologies and TRPV1 channels to investigate the putative anandamide membrane transporter. (Articolo in rivista) (literal)

Anno

• 2010-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1371/journal.pone.0010239 (literal)

Alternative label

• Ligresti A; De Petrocellis L; Hernán Pérez de la Ossa D; Aberturas R; Cristino L; Moriello AS; Finizio A; Gil ME; Torres AI; Molpeceres J; Di Marzo V (2010)
  Exploiting nanotechnologies and TRPV1 channels to investigate the putative anandamide membrane transporter.
  in PloS one
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Ligresti A; De Petrocellis L; Hernán Pérez de la Ossa D; Aberturas R; Cristino L; Moriello AS; Finizio A; Gil ME; Torres AI; Molpeceres J; Di Marzo V (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

• ISI IF (2010) 4.411 Numero Citazioni 8 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0010239 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 5 (literal)

Rivista

• PloS one (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• articlee10239pp12 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 4 (literal)

Note

• ISI Web of Science (WOS) (literal)
• Scopu (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Institute of Biomolecular Chemistry and Institute of Cybernetics, Consiglio Nazionale delle Ricerche (CNR), Pozzuoli, Italy Department of Pharmacy and Pharmaceutical Technology, School of Pharmacy, Complutense University, Madrid, Spain Department of Pharmacy and Pharmaceutical Technology, School of Pharmacy, Alcalá University, Madrid, Spain (literal)

Titolo

• Exploiting nanotechnologies and TRPV1 channels to investigate the putative anandamide membrane transporter. (literal)

Abstract

• Background: Considerable efforts have been made to characterize the pathways regulating the extracellular levels of the endocannabinoid anandamide. However, none of such pathways has been so argued as the existence of a carrier-mediated transport of anandamide across the membrane. Apart from the lack of molecular evidence for such a carrier, the main reasons of this controversy lie in the methodologies currently used to study anandamide cellular uptake. Furthermore, the main evidence in favor of the existence of an \"anandamide transporter\" relies on synthetic inhibitors of this process, the selectivity of which has been questioned. Methodology/Principal Findings: We used the cytosolic binding site for anandamide on TRPV1 channels as a biosensor to detect anandamide entry into cells, and exploited nanotechnologies to study anandamide membrane transport into intact TRPV1-overexpressing HEK-293 cells. Both fluorescence and digital holographic (DH) quantitative phase microscopy were used to study TRPV1 activation. Poly-?-caprolactone nanoparticles (PCL-NPs) were used to incorporate anandamide, which could thus enter the cell and activate TRPV1 channels bypassing any possible specific protein(s) involved in the uptake process. We reasoned that in the absence of such protein(s), pharmacological tools previously shown to inhibit the \"anandamide transporter\" would affect in the same way the uptake of anandamide and PCL-NP-anandamide, and hence the activation of TRPV1. However, when masked into PCL-NPs, anandamide cellular uptake became much less sensitive to these agents, although it maintained the same pharmacokinetics and pharmacodynamics as that of \"free\" anandamide. Conclusions: We found here that several agents previously reported to inhibit anandamide cellular uptake lose their efficacy when anandamide is prevented from interacting directly with plasma membrane proteins, thus arguing in favor of the specificity of such agents for the putative \"anandamide transporter\", and of the existence of such mechanism. (literal)

Prodotto di

• Meccanismi molecolari di trasmisssione e trasduzione dei segnali cellulari: recettori cannabici e TRP nei processi fisiopatologici (MD.P01.025.001) (Modulo)
• Struttura, funzione e \"profiling\" di endocannabinoidi e ammidi bioattive di acidi grassi implicati nelle patologie umane (PM.P01.012.001) (Modulo)
• Istituto di cibernetica \"Edoardo Caianiello\" (ISASI) (Istituto)
• Istituto di chimica biomolecolare (ICB) (Istituto)

Autore CNR

• VINCENZO DI MARZO (Unità di personale interno)
• LUIGIA CRISTINO (Persona)
• LUCIANO DE PETROCELLIS (Persona)
• ANIELLO SCHIANO MORIELLO (Persona)
• ALESSIA LIGRESTI (Persona)

Insieme di parole chiave

• Keywords of "Exploiting nanotechnologies and TRPV1 channels to investigate the putative anandamide membrane transporter." (Insieme di parole chiave)

Incoming links:

Autore CNR di

• ALESSIA LIGRESTI (Persona)
• LUIGIA CRISTINO (Persona)
• VINCENZO DI MARZO (Unità di personale interno)
• LUCIANO DE PETROCELLIS (Persona)
• ANIELLO SCHIANO MORIELLO (Persona)

Prodotto

• Istituto di cibernetica \"Edoardo Caianiello\" (ISASI) (Istituto)
• Istituto di chimica biomolecolare (ICB) (Istituto)
• Struttura, funzione e \"profiling\" di endocannabinoidi e ammidi bioattive di acidi grassi implicati nelle patologie umane (PM.P01.012.001) (Modulo)
• Meccanismi molecolari di trasmisssione e trasduzione dei segnali cellulari: recettori cannabici e TRP nei processi fisiopatologici (MD.P01.025.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• PloS one (Rivista)

Insieme di parole chiave di

• Keywords of "Exploiting nanotechnologies and TRPV1 channels to investigate the putative anandamide membrane transporter." (Insieme di parole chiave)