Olive variety identification by ligation detection reaction in a universal array format (Articolo in rivista)

Type
Label
  • Olive variety identification by ligation detection reaction in a universal array format (Articolo in rivista) (literal)
Anno
  • 2007-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.jbiotec.2007.01.025 (literal)
Alternative label
  • Clarissa Consolandi; Luisa Palmieri; Silvia Doveri, Elena Maestri; Nelson Marmiroli; Silvia Reale; David Lee; Luciana Baldoni; Nicola Tosti; Marco Severgnini; Gianluca De Bellis; Bianca Castiglioni (2007)
    Olive variety identification by ligation detection reaction in a universal array format
    in Journal of biotechnology; Academic Press Elsevier, Amsterdam (Paesi Bassi)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Clarissa Consolandi; Luisa Palmieri; Silvia Doveri, Elena Maestri; Nelson Marmiroli; Silvia Reale; David Lee; Luciana Baldoni; Nicola Tosti; Marco Severgnini; Gianluca De Bellis; Bianca Castiglioni (literal)
Pagina inizio
  • 565 (literal)
Pagina fine
  • 574 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 129 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
  • Pubblicazione scientifica riguardante lo sviluppo di un microarray per l’identificazione di cultivar di olivo. (literal)
Note
  • PubMe (literal)
  • ISI Web of Science (WOS) (literal)
  • Scopu (literal)
  • Google Scholar (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Clarissa Consolandi, Marco Severgnini; Gianluca De Bellis - ITB-CNR, Milano; Luisa Palmieri, Elena Maestri, Nelson Marmiroli - Università di Parma;Silvia Doveri, Silvia Reale, David Lee - NIAB, Cambridge, UK; Luciana Baldoni, Nicola Tosti - IGV-CNR, Perugia; - Bianca Castiglioni - IBBA-CNR, Milano (literal)
Titolo
  • Olive variety identification by ligation detection reaction in a universal array format (literal)
Abstract
  • Molecular methodologies are increasingly being developed to assess the origin and authenticity of raw organic materials and processed food products. Here we describe the application of a microarray-based assay for single nucleotide polymorphisms (SNPs) identification in olive cultivars. The assay distinguishes alleles in a ligation detection reaction (LDR), with subsequent fluorescent detection by hybridization on a universal array (UA). The LDR-UA approach was used to detect 17 SNPs in olive genomic sequences previously amplified by PCR from fresh olive leaves. Genotype calls obtained with the LDR-UA were in full agreement with those determined by direct sequencing. The panel of 17 SNPs is sufficient to discriminate 49 olive varieties selected among the most widely cultivated for olive oil production in the Mediterranean area. (literal)
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