Osmotic and aging effects in caviar oocytes throughout water and lipid changes assessed by H-1 NMR T-1 and T-2 relaxation and MRI (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Osmotic and aging effects in caviar oocytes throughout water and lipid changes assessed by H-1 NMR T-1 and T-2 relaxation and MRI (Articolo in rivista) (literal)

Anno

• 2007-01-01T00:00:00+01:00 (literal)

Alternative label

• Gussoni M, Greco F, Vezzoli A , Paleari MA, Moretti VM, Lanza B, Zetta L (2007)
  Osmotic and aging effects in caviar oocytes throughout water and lipid changes assessed by H-1 NMR T-1 and T-2 relaxation and MRI
  in Magnetic resonance imaging
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Gussoni M, Greco F, Vezzoli A , Paleari MA, Moretti VM, Lanza B, Zetta L (literal)

Pagina inizio

• 117 (literal)

Pagina fine

• 128 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 25 (literal)

Rivista

• Magnetic resonance imaging (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1. Università degli Studi di Milano, Dipartimento di Scienze e Tecnologie Biomediche, Segrate (MI) 2. CNR-Lab. NMR, Istituto per lo Studio delle Macromolecole, Milano 3. CNR-Istituto di Bioimmagini e di Fisiologia Molecolare, Milano 4. Dipartimento di Scienze e Tecnologie Veterinarie per la Sicurezza Alimentare, Università degli Studi, Milano 5. Istituto Sperimentale per la Elaiotecnica, Città S. Angelo (PE) Zetta L, Greco F, ISMAC CNR (literal)

Titolo

• Osmotic and aging effects in caviar oocytes throughout water and lipid changes assessed by H-1 NMR T-1 and T-2 relaxation and MRI (literal)

Abstract

• By combining NMR relaxation spectroscopy and magnetic resonance imaging techniques, unsalted (us) and salted (s) caviar (Acipenser transmontanus) oocytes were characterized over a storage period of up to 90 days. The aging and the salting effects on the two major cell constituents, water and lipids, were separately assessed. T(1) and T(2) decays were interpreted by assuming a two-site exchange model. At Day 0, two water compartments that were not in fast exchange were identified by the T(1) relaxation measurements on the us oocytes. In the s samples, T(1) decay was monoexponential. During the time of storage, an increment of the free water amount was found for the us oocytes, ascribed to an increased metabolism. T(1) and T(2) of the s oocytes shortened as a consequence of the osmotic stress produced by salting. Selective images showed the presence of water endowed with different regional mobility that severely changed during the storage. Lipid T(1) relaxation decays collected on us and s samples were found to be biexponential, and the T(1) values lengthened during storage. In us and s oocytes, the increased lipid mobility with the storage was ascribed to lipolysis. Selective images of us samples showed lipids that were confined to the cytoplasm for up to 60 days of storage. (literal)

Prodotto di

• Institute of molecular bioimaging and physiology (IBFM) (Istituto)
• Strutturistica NMR di proteine legate a processi patologici (PM.P01.026.003) (Modulo)
• Istituto per lo studio delle macromolecole (ISMAC) (Istituto)
• Fattori cardiovascolari e muscolari limitanti l'esercizio: ambiente e stress ossidativo (ME.P01.002.001) (Modulo)

Autore CNR

• LUCIA ZETTA (Persona)
• FULVIA GRECO (Persona)
• ALESSANDRA VEZZOLI (Persona)

Insieme di parole chiave

• Parole chiave di "Osmotic and aging effects in caviar oocytes throughout water and lipid changes assessed by H-1 NMR T-1 and T-2 relaxation and MRI" (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto per lo studio delle macromolecole (ISMAC) (Istituto)
• Institute of molecular bioimaging and physiology (IBFM) (Istituto)
• Fattori cardiovascolari e muscolari limitanti l'esercizio: ambiente e stress ossidativo (ME.P01.002.001) (Modulo)
• Strutturistica NMR di proteine legate a processi patologici (PM.P01.026.003) (Modulo)

Autore CNR di

• ALESSANDRA VEZZOLI (Persona)
• FULVIA GRECO (Persona)
• LUCIA ZETTA (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Magnetic resonance imaging (Rivista)

Insieme di parole chiave di

• Parole chiave di "Osmotic and aging effects in caviar oocytes throughout water and lipid changes assessed by H-1 NMR T-1 and T-2 relaxation and MRI" (Insieme di parole chiave)