Geobacillus toebii subsp. decanicus subsp. nov., a hydrocarbon-degrading, heavy metal resistant bacterium from hot compost (Articolo in rivista)

Type
Label
  • Geobacillus toebii subsp. decanicus subsp. nov., a hydrocarbon-degrading, heavy metal resistant bacterium from hot compost (Articolo in rivista) (literal)
Anno
  • 2006-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.2323/jgam.52.223 (literal)
Alternative label
  • Poli A.; Romano I.; Caliendo G.; Nicolaus G.; Orlando P.; de Falco A.; Lama L.; Gambacorta A.; Nicolaus B. (2006)
    Geobacillus toebii subsp. decanicus subsp. nov., a hydrocarbon-degrading, heavy metal resistant bacterium from hot compost
    in Journal of general and applied microbiology
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Poli A.; Romano I.; Caliendo G.; Nicolaus G.; Orlando P.; de Falco A.; Lama L.; Gambacorta A.; Nicolaus B. (literal)
Pagina inizio
  • 223 (literal)
Pagina fine
  • 234 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 52 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 4 (literal)
Note
  • ISI Web of Science (WOS) (literal)
  • Scopu (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Istituto di Chimica Biomolecolare (ICB), CNR, Via Campi Flegrei 34, 80078 Pozzuoli, Napoli, Italy Istituto di Biochimica delle Proteine (IBP), CNR, Napoli, Italy Istituto di Ricerche di Biologia Molecolare \"P. Angeletti\" IRBM, Pomezia, Rome, Italy Pomigliano Ambiente s.p.a., Napoli, Italy (literal)
Titolo
  • Geobacillus toebii subsp. decanicus subsp. nov., a hydrocarbon-degrading, heavy metal resistant bacterium from hot compost (literal)
Abstract
  • A thermophilic, spore-forming bacterial strain L1T was isolated from hot compost Pomigliano Environment s.p.a., Pomigliano, Naples, Italy. The strain was identified by using a polyphasic taxonomic approach. L1T resulted in an aerobic, gram-positive, rod-shaped, thermophilic with an optimum growth temperature of 68°C chemorganotrophic bacterium which grew on hydrocarbons as unique carbon and energy sources and was resistant to heavy metals. The G+C DNA content was 43.5 mol%. Phylogenetic analysis of 16S rRNA gene sequence and Random Amplified Polymorphic DNA-PCR (RAPD-PCR) analysis of L1T and related strains showed that it forms within Geobacillus toebii, a separate cluster in the Geobacillus genus. The composition of cellular fatty acids analyses by Gas-Mass Spectroscopy differed from that typical for the genus Geobacillus in that it is lacking in iso-C15 fatty acid, while iso-C16 and iso-C17 were predominant. Isolates grew on a rich complex medium at temperatures between 55-75°C and presented a doubling time (td) of 2 h and 6 h using complex media and hydrocarbon media, respectively. Among hydrocarbons tested, n-decane (2%) was the more effective to support the growth (1 g/L of wet cells). The microorganism showed resistance to heavy metal tested during the growth. Furthermore, intracellular á-galactosidase and á-glucosidase enzymatic activities were detectable in the L1T strain. Based on phenotypic, phylogenetic, fatty acid analysis and results from DNA-DNA hybridization, we propose assigning a novel subspecies of Geobacillus toebii, to be named Geobacillus toebii subsp. decanicus subsp. nov., with the type strain L1T (=DSM 17041=ATCC BAA 1004). (literal)
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