Evolutionary analysis of the APA genes in the Phaseolus genus: wild and cultivated beans species as sources of lectin-related resistance factors? (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Evolutionary analysis of the APA genes in the Phaseolus genus: wild and cultivated beans species as sources of lectin-related resistance factors? (Articolo in rivista) (literal)

Anno

• 2007-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1007/s00122-007-0622-1 (literal)

Alternative label

• Lioi L.; Galasso I.; Lanave C.; Daminati M.G.; Bollini R.; F. Sparvoli. (2007)
  Evolutionary analysis of the APA genes in the Phaseolus genus: wild and cultivated beans species as sources of lectin-related resistance factors?
  in Theoretical and applied genetics
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Lioi L.; Galasso I.; Lanave C.; Daminati M.G.; Bollini R.; F. Sparvoli. (literal)

Pagina inizio

• 959 (literal)

Pagina fine

• 970 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 115 (literal)

Rivista

• Theoretical and applied genetics (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

• Electronic supplementary material The online version of this article (doi:10.1007/s00122-007-0622-1) contains supplementary material, which is available to authorized users. (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• L. Lioi - Istituto di Genetica Vegetale, CNR, Via Amendola 165/A, 70126 Bari, Italy I. Galasso, M. G. Daminati, R. Bollini, F. Sparvoli - Istituto di Biologia e Biotecnologia Agraria, CNR, Via Bassini 15, 20133 Milano, Italy C. Lanave - Istituto di Tecnologie Biomediche, Sede di Bari, CNR, Via Amendola 122, 70126 Bari, Italy (literal)

Titolo

• Evolutionary analysis of the APA genes in the Phaseolus genus: wild and cultivated beans species as sources of lectin-related resistance factors? (literal)

Abstract

• The APA (Arcelin/Phytohemagglutinin/alpha-Amylase inhibitor) gene familyis composed of various members, present in Phaseolus species and coding for lectin and lectin-related seed proteins having the double role of storage and defense proteins. Here members of the APA family have been identified by immunological, functional, and molecular analyses and representative genes were sequenced in nine wild species of Phaseolus. All taxa possessed at least one member of the true lectin gene. No arcelin type sequences have been isolated from the species examined. Among the wild species studied, only P. costaricensis contained an alpha-amylase inhibitor (alpha-AI). In addition P. augusti, P. maculatus, P. microcarpus, and P. oligospermus showed the presence of the lectin-related alpha-amylase inhibitor-like (AIL) genes and alpha-AI activity. Data from Southern blot analysis indicated the presence of only one lectin gene in P. parvulus and P. filiformis, while an extensive gene duplication of the APA locus was found in the other Phaseolus species. Phylogenetic analysis carried out on the nucleotide sequences showed the existence of two main clusters and clearly indicated that lectin-related genes originated from a paralogous duplication event preceding the development of the ancestor to the Phaseolus genus. The finding of detectable alpha-AI activity in species containing AIL genes suggests that exploiting APA genes variability in the Phaseolus genus may represent a valuable tool to find new members that may have acquired insecticidal activities. (literal)

Prodotto di

• Biodiversità Molecolare (SV.P17.006.001) (Modulo)
• Biotecnologie agro-industriali per il miglioramento genetico, il rispetto dell'ambiente e la tutela dei prodotti, la produzione di proteine e metaboliti di interesse alimentare, salutistico e farmacologico. (AG.P01.004.001) (Modulo)
• Istituto di Bioscienze e Biorisorse (IBBR) (Istituto)
• Institute of agricultural biology and biotechnology (IBBA) (Istituto)

Autore CNR

• CECILIA LANAVE (Persona)
• FRANCESCA SPARVOLI (Persona)
• LUCIA LIOI (Unità di personale interno)
• MARIA GLORIA DAMINATI (Unità di personale interno)
• ROBERTO BOLLINI (Persona)
• INCORONATA GALASSO (Persona)

Insieme di parole chiave

• Keywords of "Evolutionary analysis of the APA genes in the Phaseolus genus: wild and cultivated beans species as sources of lectin-related resistance factors?" (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of agricultural biology and biotechnology (IBBA) (Istituto)
• Istituto di Bioscienze e Biorisorse (IBBR) (Istituto)
• Biodiversità Molecolare (SV.P17.006.001) (Modulo)
• Biotecnologie agro-industriali per il miglioramento genetico, il rispetto dell'ambiente e la tutela dei prodotti, la produzione di proteine e metaboliti di interesse alimentare, salutistico e farmacologico. (AG.P01.004.001) (Modulo)

Autore CNR di

• INCORONATA GALASSO (Persona)
• CECILIA LANAVE (Persona)
• FRANCESCA SPARVOLI (Persona)
• ROBERTO BOLLINI (Persona)
• MARIA GLORIA DAMINATI (Unità di personale interno)
• LUCIA LIOI (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Theoretical and applied genetics (Rivista)

Insieme di parole chiave di

• Keywords of "Evolutionary analysis of the APA genes in the Phaseolus genus: wild and cultivated beans species as sources of lectin-related resistance factors?" (Insieme di parole chiave)