Bioactive modification of silicon surface using self-assembled hydrophobins from Pleurotus ostreatus (Articolo in rivista)

Type
Label
  • Bioactive modification of silicon surface using self-assembled hydrophobins from Pleurotus ostreatus (Articolo in rivista) (literal)
Anno
  • 2009-01-01T00:00:00+01:00 (literal)
Alternative label
  • De Stefano L, Rea I, De Tommasi E, Rendina I, Rotiroti L, Giocondo M, Longobardi S, Armenante A, Giardina P (2009)
    Bioactive modification of silicon surface using self-assembled hydrophobins from Pleurotus ostreatus
    in The European physical journal. E, Soft matter (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • De Stefano L, Rea I, De Tommasi E, Rendina I, Rotiroti L, Giocondo M, Longobardi S, Armenante A, Giardina P (literal)
Pagina inizio
  • 181 (literal)
Pagina fine
  • 185 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 30 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1. CNR, Unit Naples, Inst Microelect & Microsyst, I-80131 Naples, Italy 2. Univ Naples Federico 2, Dept Organ Chem & Biochem, I-80126 Naples, Italy 3. CNR, INFM, LICRYL, I-87036 Cosenza, Italy (literal)
Titolo
  • Bioactive modification of silicon surface using self-assembled hydrophobins from Pleurotus ostreatus (literal)
Abstract
  • A crystalline silicon surface can be made biocompatible and chemically stable by a self-assembled biofilm of proteins, the hydrophobins (HFBs) purified from the fungus Pleurotus ostreatus. The protein-modified silicon surface shows an improvement in wettability and is suitable for immobilization of other proteins. Two different proteins were successfully immobilized on the HFBs-coated chips: the bovine serum albumin and an enzyme, a laccase, which retains its catalytic activity even when bound on the chip. Variable-angle spectroscopic ellipsometry (VASE), water contact angle (WCA), and fluorescence measurements demonstrated that the proposed approach in silicon surface bioactivation is a feasible strategy for the fabrication of a new class of hybrid devices. (literal)
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