Dimerizable redox-sensitive triazine-based cationic lipids for in vitro gene delivery (Articolo in rivista)

Type
Label
  • Dimerizable redox-sensitive triazine-based cationic lipids for in vitro gene delivery (Articolo in rivista) (literal)
Anno
  • 2007-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1002/jgm.1186 (literal)
Alternative label
  • Candiani G., Frigerio M., Viani F., Verpelli C., Sala C., Chiamenti L., Zaffaroni N., Folini M., Sani M., Panzeri W., Zanda M. (2007)
    Dimerizable redox-sensitive triazine-based cationic lipids for in vitro gene delivery
    in ChemMedChem (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Candiani G., Frigerio M., Viani F., Verpelli C., Sala C., Chiamenti L., Zaffaroni N., Folini M., Sani M., Panzeri W., Zanda M. (literal)
Pagina inizio
  • 292 (literal)
Pagina fine
  • 296 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://onlinelibrary.wiley.com/doi/10.1002/cmdc.200600267/pdf (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 2 (literal)
Rivista
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  • 6 (literal)
Note
  • Scopus (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Candiani, G.ab , Pezzoli, D.a, Cabras, M.a, Ristori, S.c, Pellegrini, C.a, Kajaste-Rudnitski, A.d, Vicenzi, E.d, Sala, C.e, Zanda, M.a a CNR, Istituto di Chimica del Riconoscimento Molecolare, via Mancinelli 7, 20131 Milan, Italy b BioCell, Department of Chemistry Materials and Chemical Engineering G. Natta, Politecnico di Milano, via Mancinelli 7, 20131 Milan, Italy c Department of Chemistry, University of Florence, Sesto Fiorentino, Italy d Viral Pathogens and Biosafety Unit, San Raffaele Scientific Institute, Milan, Italy e CNR, Institute of Neuroscience Cellular and Molecular Pharmacology, Milan, Italy (literal)
Titolo
  • Dimerizable redox-sensitive triazine-based cationic lipids for in vitro gene delivery (literal)
Abstract
  • Background: Despite the use of currently optimized lipofection conditions, including transfection in serum-depleted media, the efficiency of gene transfer is low and high transfection rates often induce cytotoxicity. A lipid formulation with transfection efficiency not inhibited by serum would provide an advance towards in vivo applications. Methods: We explored the ability of the cationic lipid SH-14 to dimerize upon DNA and form lipoplexes, and potentially release nucleic acids in the intracellular reducing milieu. We investigated the critical micelle-forming concentration of SH-14 and its intrinsic toxicity, size and Zeta potential measurements, the in vitro cytotoxicity of SH-14/ plasmid DNA lipoplexes and their ability to transfect cells. Results: Among all the charge ratios (CR, +/-) tested, lipoplexes at CR 10 with a mean diameter of 295 nm and a surface charge of +20 mV, exhibited the best compromise between transfection efficiency and tolerability. SH-14 presented the same cytotoxicity level whether alone or complexed in lipoplexes. Lipofections carried out in serum-free medium shared a transfection efficiency, on average, of 40% and a cytotoxicity of 38%. An increase of 73% in transfection efficiency and 24% in cell viability were obtained, extending lipofection over 48 h in complete-medium. Moreover, when serum concentration was increased from 10% to 50%, a three-fold increase in plasmid dose led to more than 72% of cells being transfected with almost no sign of cytotoxicity. Conclusions: Overall, SH-14 presents good potential as a novel transfection reagent to be used in the presence of serum. (literal)
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