http://www.cnr.it/ontology/cnr/individuo/prodotto/ID167813
Ligase detection reaction (LDR) and universal array (Zip Code): application to DNA genotyping (Articolo in rivista)
- Type
- Label
- Ligase detection reaction (LDR) and universal array (Zip Code): application to DNA genotyping (Articolo in rivista) (literal)
- Anno
- 2002-01-01T00:00:00+01:00 (literal)
- Alternative label
De Bellis G., Castiglioni B., Bordoni R., Mezzelani A., Rizzi E., Frosini A., Busti E., Consolandi C., Rossi Bernardi L., Battaglia C. (2002)
Ligase detection reaction (LDR) and universal array (Zip Code): application to DNA genotyping
in Minerva biotecnologica (Testo stamp.)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- De Bellis G., Castiglioni B., Bordoni R., Mezzelani A., Rizzi E., Frosini A., Busti E., Consolandi C., Rossi Bernardi L., Battaglia C. (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Titolo
- Ligase detection reaction (LDR) and universal array (Zip Code): application to DNA genotyping (literal)
- Abstract
- The Universal DNA microarray approach has been proposed for setting up
several different DNA analysis. Such an approach is based on an arrayed
set of oligonucleotides (the zipcode sequences) having common
thermodinamic behaviour but being unrelated to any specific DNA sequence
under study. This procedure, that can be combined to ligation, extension
or minisequencing, requires the design of a set of probes specific for
each target sequence (mutations, polymorphism, etc.) appended with
complementary ZipCode (cZipCode) sequences. These probes are reacted
against a proper target in solution in a multiplexed format using the
above mentioned enzymatic reactions. The resulting products, bearing their
own cZipCode are addressed to the location on the microarray where the
corresponding ZipCode sequence has been spotted. Such an array is
therefore Universal being unrelated to a specific molecular analysis.
Here we present the set up and optimization of this procedure and its
application to bacterial discrimination. Four presentations follow
demonstrating the flexibility of the Universal array format in detecting
pathogens and GMOs, monitoring bacterial diversity and typing SNPs. (literal)
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