http://www.cnr.it/ontology/cnr/individuo/prodotto/ID167705
Low cell dosage of lymphoblastoid human cell lines EBV(+) is associated to chronic hepatitis in a minority of inoculated Nu/Nu mice (Articolo in rivista)
- Type
- Label
- Low cell dosage of lymphoblastoid human cell lines EBV(+) is associated to chronic hepatitis in a minority of inoculated Nu/Nu mice (Articolo in rivista) (literal)
- Anno
- 2002-01-01T00:00:00+01:00 (literal)
- Alternative label
Bertolini L. 1, Iacovacci S. 2, Bosman C. 3, Carloni G. 4, Monaco V. 5, Bangrazi C. 6, Serafino A. 7, Gualandi G. 8, Prantera G. 9, Fruscalzo A. 10 (2002)
Low cell dosage of lymphoblastoid human cell lines EBV(+) is associated to chronic hepatitis in a minority of inoculated Nu/Nu mice
in Journal of medical virology (Print)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Bertolini L. 1, Iacovacci S. 2, Bosman C. 3, Carloni G. 4, Monaco V. 5, Bangrazi C. 6, Serafino A. 7, Gualandi G. 8, Prantera G. 9, Fruscalzo A. 10 (literal)
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- Impact Factor 2002: 2.881
Dati basati su tecniche di PCR, ed ibridazione in situ dimostrano
una stretta associazione tra le lesioni epatiche e l'infezione precedente da EBV. (literal)
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- Rivista
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- Viene descritto un modello animale di epatite cronica associata all'infezione da virus di Epstein-Barr (EBV) ottenuto nel topo immunodeprivato mediante inoculazione di cellule della linea linfoblastoide umana positiva per l'EBV. (literal)
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- 1,IBC CNR, 2,4,5,7 INMM CNR,
6, UNI Roma Tor Vergata,
8,9 UNI Viterbo La Tuscia,
10, CAN CNR, (literal)
- Titolo
- Low cell dosage of lymphoblastoid human cell lines EBV(+) is associated to chronic hepatitis in a minority of inoculated Nu/Nu mice (literal)
- Abstract
- It has been suggested that an atypical course of primary infection by EBV and the reactivation of EBV infection in transplanted patients may induce hepatitis. We explored the possibility to dissect the infectious activity from the ability to promote B lymphocyte proliferation in vivo by injecting in nu/nu mice a low number (2 x 10(6)-0.05 x 10(6)) of cells from CE a normal human bone marrow-derived B cell line. This line carries an endogenous EBV in episomal and linear forms. Twenty nu/nu mice were inoculated subcutaneously with the B cell line CE and a matched group with the cell line RAG obtained by EBV in vitro infection of normal human peripheral blood. The mice injected with the CE line did not develop a lymphoproliferative disease, but 5 of them displayed typical histopathological lesions of chronic hepatitis without involvement of other organs. Similar results were obtained in 2 out of 20 animals in the RAG group. A close association between liver lesions and a previous EBV infection, by putative circulating B lymphoblastoid cells releasing their EBV, was established by PCR and by in situ hybridization with BamHI \"W\" DNA probe. This latter probe detected the presence of about 15% of positive cells only in affected livers. In addition, the rare detection in some hepatocytes of \"A\" type Cowdry bodies would suggest the occurrence of continuous EBV replication although at a very low level. These data show that we succeeded in dissecting the infectious from the proliferative activity of the endogenous EBV carrier CE cell line. This provides in addition a promising model for chronic EBV-associated hepatitis. (literal)
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