The dawn of dominance by the mature domain in tRNA splicing (Articolo in rivista)

Type
Label
  • The dawn of dominance by the mature domain in tRNA splicing (Articolo in rivista) (literal)
Anno
  • 2007-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1073/pnas.0705537104 (literal)
Alternative label
  • Tocchini-Valentini G.D.; Fruscoloni P.; Tocchini-Valentini G.P. (2007)
    The dawn of dominance by the mature domain in tRNA splicing
    in Proceedings of the National Academy of Sciences of the United States of America
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Tocchini-Valentini G.D.; Fruscoloni P.; Tocchini-Valentini G.P. (literal)
Pagina inizio
  • 12300 (literal)
Pagina fine
  • 12305 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://www.pnas.org/content/104/30/12300.long (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 104 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
  • Pubblicazione su rivista internazionale (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Istituto di Biologia Cellulare, Consiglio Nazionale delle Ricerche, Campus A, Buzzati-Traverso, Via Ramarini 32, Monterotondo Scalo, 00016 Rome, Italy; and Istituto di Genetica Molecolare, Consiglio Nazionale delle Ricerche, Via Abbiategrasso 207, I-27100 Pavia, Italy (literal)
Titolo
  • The dawn of dominance by the mature domain in tRNA splicing (literal)
Abstract
  • The relationship between enzyme architecture and substrate specificity among archaeal pre-tRNA splicing endonucleases has been investigated more deeply, by using biochemical assays and model building. The enzyme from Archeoglobus fulgidus (AF) is particularly interesting: it cleaves the bulge-helix-bulge target without requiring the mature tRNA domain, but, when the target is a bulge-helix-loop, the mature domain is required. A model of AF based on its electrostatic potential shows three polar patches interacting with the pre-tRNA substrate. A simple deletion mutant of the AF endonuclease lacking two of the three polar patches no longer cleaves the bulge-helix-loop substrate with or without the mature domain. This single deletion shows a possible path for the evolution of eukaryal splicing endonucleases from the archaeal enzyme. (literal)
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