DNA sequence heterogeneity within the Epstein-Barr virus family of repeats in the latent origin of replication (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• DNA sequence heterogeneity within the Epstein-Barr virus family of repeats in the latent origin of replication (Articolo in rivista) (literal)

Anno

• 2001-01-01T00:00:00+01:00 (literal)

Alternative label

• Fruscalzo A., Marsili G., Busiello V., Bertolini L., Frezza D. (2001)
  DNA sequence heterogeneity within the Epstein-Barr virus family of repeats in the latent origin of replication
  in Gene (Amst.)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Fruscalzo A., Marsili G., Busiello V., Bertolini L., Frezza D. (literal)

Pagina inizio

• 165 (literal)

Pagina fine

• 173 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

• These new data on the EBV oriP genome structure could be very useful to develop more efficient vectors in the gene therapy experiments. The paper is published in a peer-reviewed international journal with an impact factor of 3.041. (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 265 (literal)

Rivista

• Gene (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto

• Pubblicazione su rivista scientifica We analyzed the length of the family of repeats in the EBV strains of several latently infected cell lines by PCR amplification. The sequence analysis revealed that each line is different both in the number and in the sequence of repeats. In the B95-8 virus, the family of repeats resulted 252 bp longer than that previously sequenced and reported in the database. The length analysis of the family of repeats can be used to characterize EBV strains by PCR. (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1 CNR (C.S. sugli Acidi Nucleici), 2 CNR (Istituto di Biologia Cellulare), 3 UNI Tor Vergata (Dipartimento di Biologia). (literal)

Titolo

• DNA sequence heterogeneity within the Epstein-Barr virus family of repeats in the latent origin of replication (literal)

Abstract

• To detect the presence of variability in the tandemly repeated sequences of the Epstein-Barr virus latent origin of replication, we analyzed the length of the family of repeats in 14 lymphoblastoid and Burkitt's lymphoma cell lines by PCR amplification. The gel electrophoresis analysis of the PCR products revealed a broad banding pattern, characteristic of each line, consisting of several fragments, sometimes smeared, of variable length. This finding was interpreted as a result of the hairpin-like structures generated by the palindrome within the family of repeats, able to originate artefacts. Since the banding pattern was different only in strictly non-correlated cell lines, we supposed that the sequence of the repeat units was polymorphic. We therefore sequenced the family of repeats in three healthy bone marrow derived lymphoblastoid cell lines carrying an endogenous EBV as well as in a B95-8 infected cell line as control. The sequence analysis revealed that each line is different both in the number and in the sequence of repeats. At the 3' end of the family of repeats the B95-8 virus was found to have a 252 bp region missing in the GenBank standard sequence. This one is probably a partial sequence since it was shorter than the control specimens obtained from different sources of B95-8 DNA analyzed by Southern blot hybridization. The length analysis of the family of repeats can be used to characterize EBV strains by PCR. (literal)

Prodotto di

• Institute of cell biology (IBC) (Istituto)
• Institute of molecular biology and pathology (IBPM) (Istituto)

Autore CNR

• ALBERTO FRUSCALZO (Persona)
• VINCENZO BUSIELLO (Persona)

Insieme di parole chiave

• Keywords of "DNA sequence heterogeneity within the Epstein-Barr virus family of repeats in the latent origin of replication" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• ALBERTO FRUSCALZO (Persona)
• VINCENZO BUSIELLO (Persona)

Prodotto

• Institute of molecular biology and pathology (IBPM) (Istituto)
• Institute of cell biology (IBC) (Istituto)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Gene (Rivista)

Insieme di parole chiave di

• Keywords of "DNA sequence heterogeneity within the Epstein-Barr virus family of repeats in the latent origin of replication" (Insieme di parole chiave)