Inhibitory properties and binding loop polymorphism in Bowman-Birk inhibitors from Phaseolus species (Articolo in rivista)

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  • Inhibitory properties and binding loop polymorphism in Bowman-Birk inhibitors from Phaseolus species (Articolo in rivista) (literal)
Anno
  • 2010-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1007/s10722-009-9491-6 (literal)
Alternative label
  • Lioi L., Galasso I., Daminati M.G., Piergiovanni A.R. (2010)
    Inhibitory properties and binding loop polymorphism in Bowman-Birk inhibitors from Phaseolus species
    in Genetic resources and crop evolution (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Lioi L., Galasso I., Daminati M.G., Piergiovanni A.R. (literal)
Pagina inizio
  • 533 (literal)
Pagina fine
  • 542 (literal)
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  • http://www.springerlink.com/content/y75n12k4624r0284/ (literal)
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  • 57 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
  • Published online 15 October 2009 DOI 10.1007/s10722-009-9491-6 DOI 10.1007/s10722-099-9491-6 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 10 (literal)
Note
  • ISI Web of Science (WOS) (literal)
  • Scopu (literal)
  • Google Scholar (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • IGV-CNR; IBBA-CNR (literal)
Titolo
  • Inhibitory properties and binding loop polymorphism in Bowman-Birk inhibitors from Phaseolus species (literal)
Abstract
  • The primary structure of the double-headed Bowman-Birk inhibitor (BBI) family and the antitryptic activity were investigated in cultivated and wild Phaseolus species. Two BBI types were identified; the first one inhibits trypsin and chymotrypsin (tc-BBI), the second one elastase and trypsin (et-BBI). Only tc-BBI was found in P. lunatus and P. parvulus, while none of BBI types, identified in this study, was found in P. leptostachyus. The deduced amino acid sequences revealed some polymorphisms within both tc-BBI and et-BBI binding loops that could affect the inhibitory activity. The trypsin inhibitor content showed a high variation with the lowest value recorded in P. lepthostachyus and the highest one observed in P. oligospermus. Southern blot analysis confirmed the absence of both BBI types in P. leptostachyus and suggests that in P. coccineus, P. dumosus and P. costaricensis, the two genes were clustered in a narrow genomic region of 1.3kbp. (literal)
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