Identification of hepatotoxin-producing cyanobacteria by DNA-chip (Articolo in rivista)

Type
Label
  • Identification of hepatotoxin-producing cyanobacteria by DNA-chip (Articolo in rivista) (literal)
Anno
  • 2008-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1111/j.1462-2920.2007.01488.x (literal)
Alternative label
  • Anne Rantala; Ermanno Rizzi; Bianca Castiglioni; Gianluca De Bellis; Karina Sivonen (2008)
    Identification of hepatotoxin-producing cyanobacteria by DNA-chip
    in Environmental microbiology (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Anne Rantala; Ermanno Rizzi; Bianca Castiglioni; Gianluca De Bellis; Karina Sivonen (literal)
Pagina inizio
  • 653 (literal)
Pagina fine
  • 664 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 10 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
  • Impact Factor della rivista: 4.929 Environ Microbiol. 2008 Mar;10(3):653-64. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
  • Pubblicazione scientifica riguardante l’utilizzo di un DNA chip per il monitoraggio di batteri epatotossici in Finlandia (literal)
Note
  • ISI Web of Science (WOS) (literal)
  • Scopus (literal)
  • PubMed (literal)
  • Google Scholar (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Anne Rantala, Karina Sivonen - Department of Applied Chemistry and Microbiology, University of Helsinki, Finlandia; Ermanno Rizzi, Gianluca De Bellis - ITB-CNR, Segrate (Mi); Bianca Castiglioni - IBBA-CNR, Milano (literal)
Titolo
  • Identification of hepatotoxin-producing cyanobacteria by DNA-chip (literal)
Abstract
  • We developed a new tool to detect and identify hepatotoxin-producing cyanobacteria of the genera Anabaena, Microcystis, Planktothrix, Nostoc and Nodularia. Genus-specific probe pairs were designed for the detection of the microcystin (mcyE) and nodularin synthetase genes (ndaF) of these five genera to be used with a DNA-chip. The method couples a ligation detection reaction, in which the polymerase chain reaction (PCR)-amplified mcyE/ndaF genes are recognized by the probe pairs, with a hybridization on a universal microarray. All the probe pairs specifically detected the corresponding mcyE/ndaF gene sequences when DNA from the microcystin- or nodularin-producing cyanobacterial strains were used as template in the PCR. Furthermore, the strict specificity of detection enabled identification of the potential hepatotoxin producers. The specificity, sensitivity and ability of the DNA-chip in simultaneously detecting all the main hepatotoxin producers make this method suitable for highthroughput analysis and monitoring of environmental samples. (literal)
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