Voltage-induced morphological modifications in oocyte membranes containing exogenous K(+) channels studied by electrochemical scanning force microscopy (Articolo in rivista)

Type
Label
  • Voltage-induced morphological modifications in oocyte membranes containing exogenous K(+) channels studied by electrochemical scanning force microscopy (Articolo in rivista) (literal)
Anno
  • 2008-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1002/jemt.20552 (literal)
Alternative label
  • Alessandrini A; Gavazzo P; Picco C; Facci P (2008)
    Voltage-induced morphological modifications in oocyte membranes containing exogenous K(+) channels studied by electrochemical scanning force microscopy
    in Microscopy research and technique (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Alessandrini A; Gavazzo P; Picco C; Facci P (literal)
Pagina inizio
  • 274 (literal)
Pagina fine
  • 278 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 71 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 4 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • \"[Alessandrini, Andrea; Facci, Paolo] CNR, Natl Inst Phys Matter, Ctr Nanostruct & Biosyst Surfaces S3, Modena, Italy; [Alessandrini, Andrea] Univ Modena, Dept Phys, I-41100 Modena, Italy; [Gavazzo, Paola; Picco, Cristiana] CNR, Inst Biophys, Genoa, Italy (literal)
Titolo
  • Voltage-induced morphological modifications in oocyte membranes containing exogenous K(+) channels studied by electrochemical scanning force microscopy (literal)
Abstract
  • We report on a novel use of electrochemical scanning force microscopy (SFM) for the investigation of morphological modifications occurring in plasma membranes containing voltage-gated ion channels, on membrane potential variation. Membrane patches of Xenopus laevis oocytes microinjected with exogenous KAT1 cRNA, deposited by a stripping method at the surface of a derivatized gold film in inside-out configuration, have been imaged by SFM in an electrochemical cell. A potentiostat was used to maintain a desired potential drop across the membrane. Performing imaging at potential values corresponding to open (- 120 mV) and closed (+ 20 mV) states for KAT1, morphological differences in localized sample zones were observed. Particularly, cross-shaped features involving a significant membrane portion appear around putative channel locations. The reported approach constitutes the first demonstration of an SPM-based experimental technique suitable to investigate the rearrangements occurring to the plasma membrane containing voltage-gated channels on transmembrane potential variation. (literal)
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