Unfolding time distribution of GFP by single molecule fluorescence spectroscopy (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Unfolding time distribution of GFP by single molecule fluorescence spectroscopy (Articolo in rivista) (literal)

Anno

• 2006-01-01T00:00:00+01:00 (literal)

Alternative label

• Chirico, G; Cannone, F; Diaspro, A (2006)
  Unfolding time distribution of GFP by single molecule fluorescence spectroscopy
  in European biophysics journal
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Chirico, G; Cannone, F; Diaspro, A (literal)

Pagina inizio

• 663 (literal)

Pagina fine

• 674 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 35 (literal)

Rivista

• European biophysics journal (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Univ Milano Bicocca, Dept Phys, I-20126 Milan, Italy; Univ Genoa, LAMBS, MicroScoBio Res Ctr, I-16146 Genoa, Italy; Univ Genoa, Dept Phys, I-16146 Genoa, Italy; CNR, INFM, Genoa, Italy; IFOM, FIRC Inst Mol Oncol Fdn, I-20139 Milan, Italy (literal)

Titolo

• Unfolding time distribution of GFP by single molecule fluorescence spectroscopy (literal)

Abstract

• We have studied the unfolding of single molecules of GFP-mut2 mutant trapped in wet silica gels in a wide range of GuHCl concentration. After the addition of denaturant, the number of fluorescent molecules decreases with unfolding rates (of the order of 0.01 min(-1)) that are in very good agreement with bulk fluorescence and circular dichroism data. Unexpectedly, single molecule experiments show rare fluctuations in the number of fluorescent proteins at equilibrium. On the other hand, although a first approximate description of the number decays can be reasonably performed by single exponential functions, the distributions of the single molecule unfolding times show a maximum at times congruent to 50-100 min up to the denaturation midpoint concentration of [GuHCl] congruent to 2.5 M. A theoretical analysis of the distributions indicates that this feature is a fingerprint of the competition between unfolding and refolding processes when the protein is very far from the midpoint denaturant concentration. (literal)

Prodotto di

• Istituto di biofisica (IBF) (Istituto)
• Processi di membrana nella comunicazione intra- ed inter-cellulare (MD.P01.001.001) (Modulo)
• Fotonica ultrabreve dall'infrarosso al EUV: applicazioni a materiali e dispositivi per ICT, magnetismo, biomedicina e nanoscienze (MD.P03.008.001) (Modulo)

Autore CNR

• ALBERTO DIASPRO (Persona)
• GIUSEPPE CHIRICO (Persona)

Insieme di parole chiave

• Parole chiave di "Unfolding time distribution of GFP by single molecule fluorescence spectroscopy" (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto di biofisica (IBF) (Istituto)
• Fotonica ultrabreve dall'infrarosso al EUV: applicazioni a materiali e dispositivi per ICT, magnetismo, biomedicina e nanoscienze (MD.P03.008.001) (Modulo)
• Processi di membrana nella comunicazione intra- ed inter-cellulare (MD.P01.001.001) (Modulo)

Autore CNR di

• ALBERTO DIASPRO (Persona)
• GIUSEPPE CHIRICO (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• European biophysics journal (Rivista)

Insieme di parole chiave di

• Parole chiave di "Unfolding time distribution of GFP by single molecule fluorescence spectroscopy" (Insieme di parole chiave)