Impairment of circulating endothelial progenitors in Down syndrome (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Impairment of circulating endothelial progenitors in Down syndrome (Articolo in rivista) (literal)

Anno

• 2010-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1186/1755-8794-3-40 (literal)

Alternative label

• Costa V.; Sommese L.; Casamassimi A.; Colicchio R.; Angelini C.; Marchesano V.; Milone L.; Farzati B.; Giovane A.; Fiorito C.; Rienzo M.; Picardi M.; Avallone B.; Corsi M.M.; Sarubbi B.; Calabrò R.; Salvatore P.; Ciccodicola A. and Napoli C. (2010)
  Impairment of circulating endothelial progenitors in Down syndrome
  in BMC genomics
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Costa V.; Sommese L.; Casamassimi A.; Colicchio R.; Angelini C.; Marchesano V.; Milone L.; Farzati B.; Giovane A.; Fiorito C.; Rienzo M.; Picardi M.; Avallone B.; Corsi M.M.; Sarubbi B.; Calabrò R.; Salvatore P.; Ciccodicola A. and Napoli C. (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 3 (literal)

Rivista

• BMC genomics (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

• PMID: 20836844 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 13 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 40 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• IGB CNR, Inst Genet & Biophys Buzzati Traverso, Naples, Italy Univ Naples 2, Microbiol Sect, Dept Expt Med, Sch Med 1, Naples, Italy Univ Naples 2, Sch Med 1, Dept Gen Pathol, Naples, Italy Univ Naples 2, Sch Med 1, Excellence Res Ctr Cardiovasc Dis, Naples, Italy IRCCS Fdn SDN, Naples, Italy CNR, Ist Applicaz Calcolo Mauro Picone, I-80125 Naples, Italy Univ Naples Federico 2, Dept Biol Sci, Naples, Italy Univ Naples 2, Dept Biochem & Biophys, Naples, Italy IRCCS Multimed, Milan, Italy Univ Naples Federico 2, Dept Biochem & Med Biotechnol, Naples, Italy Univ Milan, Inst Gen Pathol, Sect Clin Pathol, Fac Med, Milan, Italy Second Univ Naples Monaldi Hosp, Dept Cardiol, Naples, Italy Univ Naples Federico 2, Dept Cellular & Mol Biol & Pathol L Califano, Naples, Italy Univ Naples Federico 2, Sch Biotechnol Sci, Naples, Italy (literal)

Titolo

• Impairment of circulating endothelial progenitors in Down syndrome (literal)

Abstract

• Background: Pathological angiogenesis represents a critical issue in the progression of many diseases. Down syndrome is postulated to be a systemic anti-angiogenesis disease model, possibly due to increased expression of anti-angiogenic regulators on chromosome 21. The aim of our study was to elucidate some features of circulating endothelial progenitor cells in the context of this syndrome. Methods: Circulating endothelial progenitors of Down syndrome affected individuals were isolated, in vitro cultured and analyzed by confocal and transmission electron microscopy. ELISA was performed to measure SDF-1 alpha plasma levels in Down syndrome and euploid individuals. Moreover, qRT-PCR was used to quantify expression levels of CXCL12 gene and of its receptor in progenitor cells. The functional impairment of Down progenitors was evaluated through their susceptibility to hydroperoxide-induced oxidative stress with BODIPY assay and the major vulnerability to the infection with human pathogens. The differential expression of crucial genes in Down progenitor cells was evaluated by microarray analysis. Results: We detected a marked decrease of progenitors' number in young Down individuals compared to euploid, cell size increase and some major detrimental morphological changes. Moreover, Down syndrome patients also exhibited decreased SDF-1 alpha plasma levels and their progenitors had a reduced expression of SDF-1 alpha encoding gene and of its membrane receptor. We further demonstrated that their progenitor cells are more susceptible to hydroperoxide-induced oxidative stress and infection with Bartonella henselae. Further, we observed that most of the differentially expressed genes belong to angiogenesis, immune response and inflammation pathways, and that infected progenitors with trisomy 21 have a more pronounced perturbation of immune response genes than infected euploid cells. Conclusions: Our data provide evidences for a reduced number and altered morphology of endothelial progenitor cells in Down syndrome, also showing the higher susceptibility to oxidative stress and to pathogen infection compared to euploid cells, thereby confirming the angiogenesis and immune response deficit observed in Down syndrome individuals. (literal)

Prodotto di

• Algoritmi Statistici orientati al grid computing per la genetica (INT.P02.004.001) (Modulo)
• Plasticità genomica: dal genoma ai sistemi biologici (SV.P13.003.001) (Modulo)
• Approccio multidisciplinare per la definizione di networks molecolari regolanti tratti ad eredità mendeliana e multifattoriale (SV.P18.005.001) (Modulo)
• Istituto di genetica e biofisica \"Adriano Buzzati Traverso\" (IGB) (Istituto)
• Istituto per le applicazioni del calcolo \"Mauro Picone\" (IAC) (Istituto)

Autore CNR

• VALERIO COSTA (Unità di personale esterno)
• ALFREDO CICCODICOLA (Persona)
• CLAUDIA ANGELINI (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "Impairment of circulating endothelial progenitors in Down syndrome" (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto per le applicazioni del calcolo \"Mauro Picone\" (IAC) (Istituto)
• Istituto di genetica e biofisica \"Adriano Buzzati Traverso\" (IGB) (Istituto)
• Approccio multidisciplinare per la definizione di networks molecolari regolanti tratti ad eredità mendeliana e multifattoriale (SV.P18.005.001) (Modulo)
• Algoritmi Statistici orientati al grid computing per la genetica (INT.P02.004.001) (Modulo)
• Plasticità genomica: dal genoma ai sistemi biologici (SV.P13.003.001) (Modulo)

Autore CNR di

• CLAUDIA ANGELINI (Unità di personale interno)
• VALERIO COSTA (Unità di personale esterno)
• ALFREDO CICCODICOLA (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• BMC genomics (Rivista)

Insieme di parole chiave di

• Parole chiave di "Impairment of circulating endothelial progenitors in Down syndrome" (Insieme di parole chiave)