Porous silicon-based optical microsensor for the detection of L-glutamine (Articolo in rivista)

Type
Label
  • Porous silicon-based optical microsensor for the detection of L-glutamine (Articolo in rivista) (literal)
Anno
  • 2006-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.bios.2005.08.012 (literal)
Alternative label
  • De Stefano L., Rotiroti L., Rendina I., Moretti L., Scognamiglio V., Rossi M., D'Auria S. (2006)
    Porous silicon-based optical microsensor for the detection of L-glutamine
    in Biosensors & bioelectronics
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • De Stefano L., Rotiroti L., Rendina I., Moretti L., Scognamiglio V., Rossi M., D'Auria S. (literal)
Pagina inizio
  • 1664 (literal)
Pagina fine
  • 1667 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 21 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Natl Council Res, Dept Naples, Inst Microelect & Microsyst, Naples, I-80131 Italy; Univ Reggio Calabria, Reggio Di Calabria, I-89060 Italy; Univ Naples, Inst Prot Biochem, Naples, I-80131 Italy (literal)
Titolo
  • Porous silicon-based optical microsensor for the detection of L-glutamine (literal)
Abstract
  • The molecular binding between the glutamine-binding protein (GlnBP) from Escherichia coli and L-glutamine (Gin) is optically transduced by means of a biosensor based on porous silicon nano-technology. The sensor operates by the measurement of the interferometric fringes in the reflectivity spectrum of a porous silicon Fabry-Perot layer. The binding event is revealed as a shift in wavelength of the fringes. Due to the hydrophobic interaction with the Si-H terminated surface of the porous silicon, the GlnBP protein, which acts as a molecular probe for Gin, penetrates and links into the pores of the porous silicon matrix. We can thus avoid any preliminary functionalization process of the porous layer surface, which is also prevented from oxidation, at least for few cycles of wet measurements. The binding of Gin to GlnBP has also been investigated at different concentration of GlnBP. (literal)
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