Processing of filamentous bacteriophage virions in antigen-presenting cells targets both HLA class I and class II peptide loading compartments. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Processing of filamentous bacteriophage virions in antigen-presenting cells targets both HLA class I and class II peptide loading compartments. (Articolo in rivista) (literal)

Anno

• 2003-01-01T00:00:00+01:00 (literal)

Alternative label

• Gaubin M., Fanutti C., Mishal Z., Durrbach A., De Berardinis P., Sartorius R., Del Pozzo G., Guardiola J., Perham R.N., Piatier-Tonneau D. (2003)
  Processing of filamentous bacteriophage virions in antigen-presenting cells targets both HLA class I and class II peptide loading compartments.
  in DNA and cell biology
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Gaubin M., Fanutti C., Mishal Z., Durrbach A., De Berardinis P., Sartorius R., Del Pozzo G., Guardiola J., Perham R.N., Piatier-Tonneau D. (literal)

Pagina inizio

• 11 (literal)

Pagina fine

• 18 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 22 (literal)

Rivista

• DNA and cell biology (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Titolo

• Processing of filamentous bacteriophage virions in antigen-presenting cells targets both HLA class I and class II peptide loading compartments. (literal)

Abstract

• Virions of filamentous bacteriophage fd are capable of displaying multiple copies of peptide epitopes and generating powerful immune responses to them. To investigate the antigen processing mechanisms in human B cell lines used as antigen presenting cells, the major coat protein (pVIII) in intact virions was fluorescently labeled, and its localization in various intracellular compartments was followed using confocal microscopy. We show that the virions were taken up and processed to yield peptides that reach both the major histocompatibility complex (MHC) class II compartment and the endoplasmic reticulum. Moreover, when exposed to bacteriophages displaying a cytotoxic T lymphocyte (CTL) epitope from the reverse transcriptase of human immunodeficiency virus type-1 (HIV-1), B cells were lysed by specific cytotoxic lymphocytes. This confirms that filamentous bacteriophage virions are capable of being taken up and processed efficiently by MHC class I and class II pathways, even in nonprofessional antigen presenting cells. These remarkable features explain, at least in part, the unexpected ability of virions displaying foreign T-cell epitopes to prime strong T-helper-dependent CTL responses. These findings have important implications for the development of peptide-based vaccines, using filamentous bacteriophage virions as scaffolds. (literal)

Prodotto di

• Istituto di genetica e biofisica \"Adriano Buzzati Traverso\" (IGB) (Istituto)
• Institute of protein biochemistry (IBP) (Istituto)

Autore CNR

• PIERGIUSEPPE DE BERARDINIS (Unità di personale interno)
• GIOVANNA DEL POZZO (Unità di personale interno)
• JOHN GUARDIOLA (Persona)
• ROSSELLA SARTORIUS (Unità di personale interno)

Incoming links:

Prodotto

• Istituto di genetica e biofisica \"Adriano Buzzati Traverso\" (IGB) (Istituto)
• Institute of protein biochemistry (IBP) (Istituto)

Autore CNR di

• PIERGIUSEPPE DE BERARDINIS (Unità di personale interno)
• GIOVANNA DEL POZZO (Unità di personale interno)
• JOHN GUARDIOLA (Persona)
• ROSSELLA SARTORIUS (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• DNA and cell biology (Rivista)