http://www.cnr.it/ontology/cnr/individuo/prodotto/ID15953
Production and characterization of a microbial glucan, synthesized by Geobacillus tepidamans V264 isolated from Bulgarian hot spring (Articolo in rivista)
- Type
- Label
- Production and characterization of a microbial glucan, synthesized by Geobacillus tepidamans V264 isolated from Bulgarian hot spring (Articolo in rivista) (literal)
- Anno
- 2009-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1016/j.carbpol.2009.01.004 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Margarita Kambourova; Rossica Mandeva; Damyana Dimova; Annarita Poli; Barbara Nicolaus; Giuseppina Tommonaro (literal)
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
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- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Bulgarian Academy of Sciences, Department of Microbiology, Acad G. Bonchev Str., Blv. 26, 1113 Sofia, Bulgaria;
Institute of Biomolecular Chemistry, ICB-CNR, Via Campi Flegrei, 34, 80078, Pozzuoli (NA), Italy (literal)
- Titolo
- Production and characterization of a microbial glucan, synthesized by Geobacillus tepidamans V264 isolated from Bulgarian hot spring (literal)
- Abstract
- The thermophilic strain producing extracellular polysaccharide (EPS) was isolated from Velingrad hot
spring, Bulgaria. StrainV264 showed 98% similarity in 16S rRNA gene sequence with Geobacillus tepidamans.
Maltose was found to be the most appropriate carbon source for exopolysaccharide production.
Maximum EPS production was reached in the early stationary phase. Highest EPS production of 111.4
mg L?1 was obtained in fermentor batch cultures at 300 rpm. The purified EPS expressed a high molecular
weight and it was very stable at high temperature, showing a degradation at 280 ?C. Chemical composition
of the bio-polymer, determined by using a high pressure anion exchange-pulsed amperometric
detector (HPAE-PAD), showed glucose as its major component. The polysaccharide was also investigated
by spectroscopic methods (1H and 13C NMR) which demonstrated the structure complexity of this biopolymer. (literal)
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