Pectin methylesterase in Citrus bergamia R.: purification, biochemical characterisation and sequence of the exon related to the enzyme active site (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Pectin methylesterase in Citrus bergamia R.: purification, biochemical characterisation and sequence of the exon related to the enzyme active site (Articolo in rivista) (literal)

Anno

• 2008-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.foodchem.2008.02.065 (literal)

Alternative label

• Laratta B.; De Masi L.; Minasi P. ; Giovane A. (2008)
  Pectin methylesterase in Citrus bergamia R.: purification, biochemical characterisation and sequence of the exon related to the enzyme active site
  in Food chemistry
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Laratta B.; De Masi L.; Minasi P. ; Giovane A. (literal)

Pagina inizio

• 829 (literal)

Pagina fine

• 837 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 110 (literal)

Rivista

• Food chemistry (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 4 (literal)

Note

• Scopu (literal)
• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• -Istituto di Chimica Biomolecolare (ICB), Consiglio Nazionale delle Ricerche (CNR), Via Campi Flegrei, 34-80078 Pozzuoli (NA), Italy -Istituto di Genetica Vegetale (IGV), Consiglio Nazionale delle Ricerche (CNR), Via Università, 133-80055 Portici (NA), Italy -Stazione Sperimentale per le Industrie delle Essenze e dei Derivati Agrumari, Via G. Tommasini, 2-89127 Reggio Calabria, Italy -Dipartimento di Biochimica e Biofisica ''F. Cedrangolo'', Seconda Universita`degli Studi di Napoli, Via S. M. di Costantinopoli, 16-80138 Napoli, Italy (literal)

Titolo

• Pectin methylesterase in Citrus bergamia R.: purification, biochemical characterisation and sequence of the exon related to the enzyme active site (literal)

Abstract

• Three forms of pectin methylesterase (PME) were purified, from bergamot fruit (Citrus bergamia R.), to homogeneity by ion-exchange and affinity chromatography. The isoforms, named PME I, PME II and PME III, according their elution order on a heparin-sepharose column, were characterized for their relative molecular mass, activity kinetic parameters and thermostability. The molecular mass was estimated to be 42 kDa for the three forms, and the apparent Km values for citrus pectin were 0.9 mg/ml for PME I and 0.5 mg/ml for PME II and PME III. The optimum pH values lie within the range 6.5-9.0, depending on salt concentration. Thermal behaviours of the three PME isoforms were studied in a temperature range from 65 °C to 80 °C with the less abundant PME I isoform showing a higher heat resistance. Moreover, the complete exon 2 sequence of PME gene was acquired (GenBank accession no. DQ458770) using a PCR based approach on well-known Citrus genomic DNA present in the NCBI database. (literal)

Prodotto di

• Istituto di chimica biomolecolare (ICB) (Istituto)

Autore CNR

• LUIGI DE MASI (Unità di personale interno)
• BRUNA LARATTA (Persona)

Insieme di parole chiave

• Parole chiave di "Pectin methylesterase in Citrus bergamia R.: purification, biochemical characterisation and sequence of the exon related to the enzyme active site" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• BRUNA LARATTA (Persona)
• LUIGI DE MASI (Unità di personale interno)

Prodotto

• Istituto di chimica biomolecolare (ICB) (Istituto)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Food chemistry (Rivista)

Insieme di parole chiave di

• Parole chiave di "Pectin methylesterase in Citrus bergamia R.: purification, biochemical characterisation and sequence of the exon related to the enzyme active site" (Insieme di parole chiave)