http://www.cnr.it/ontology/cnr/individuo/prodotto/ID15781
Metabonomic analysis of exhaled breath condensate in adults by Nuclear Magnetic Resonance spectroscopy (Articolo in rivista)
- Type
- Label
- Metabonomic analysis of exhaled breath condensate in adults by Nuclear Magnetic Resonance spectroscopy (Articolo in rivista) (literal)
- Anno
- 2008-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1183/09031936.00072408 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- de Laurentiis G.; Paris D.; Melck D.; Maniscalco M.; Marsico S.; Corso G.; Motta A.; Sofia M.; (literal)
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- Rivista
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- Note
- Scopu (literal)
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Dept of Respiratory Medicine, Azienda Ospedaliera Monaldi, University of Naples ''Federico II'';
Dept of Respiratory Medicine, Azienda Ospedaliera Monaldi, Second University of Naples;
Institute of Biomolecular Chemistry, Consiglio Nazionale delle Ricerche, Pozzuoli, Naples;
Dept of Biomedical Sciences, University of Foggia, Foggia, Italy. (literal)
- Titolo
- Metabonomic analysis of exhaled breath condensate in adults by Nuclear Magnetic Resonance spectroscopy (literal)
- Abstract
- Exhaled breath condensate (EBC) is a noninvasive method for the study of airway
lining fluid. Nuclear magnetic resonance (NMR) spectroscopy can provide biochemical profiles of
metabolites in biological samples. The aim of the present study was to validate the NMR
metabonomic analysis of EBC in adults, assessing the role of pre-analytical variables (saliva and
disinfectant contamination) and the potential clinical feasibility.
In total, 36 paired EBC and saliva samples, obtained from healthy subjects, laryngectomised
patients and chronic obstructive pulmonary disease patients, were analysed by means of 1H-NMR
spectroscopy followed by principal component analysis. The effect on EBC of disinfectant, used
for reusable parts of the condenser, was assessed after different washing procedures. To
evaluate intra-day repeatability, eight subjects were asked to collect EBC and saliva twice within
the same day.
All NMR saliva spectra were significantly different from corresponding EBC samples. EBC taken
from condensers washed with recommended procedures invariably showed spectra perturbed by
disinfectant. Each EBC sample clustered with corresponding samples of the same group, while
presenting intergroup qualitative and quantitative signal differences (94% of the total variance
within the data).
In conclusion, the nuclear magnetic resonance metabonomic approach could identify the
metabolic fingerprint of exhaled breath condensate in different clinical sets of data. Moreover,
metabonomics of exhaled breath condensate in adults can discriminate potential perturbations
induced by pre-analytical variables. (literal)
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