Serial detection of circulating tumour cells by reverse transcriptase-polymerase chain reaction assays is a marker for poor outcome in patients with malignant melanoma (Articolo in rivista)

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  • Serial detection of circulating tumour cells by reverse transcriptase-polymerase chain reaction assays is a marker for poor outcome in patients with malignant melanoma (Articolo in rivista) (literal)
Anno
  • 2006-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1186/1471-2407-6-266 (literal)
Alternative label
  • Palmieri G.; Satriano S.M.; Budroni M.; Cossu A.; Tanda F.; Canzanella S.; Caracò C.; Simeone E.; Daponte A.; Mozzillo N.; Comella G.; Castello G.; Ascierto P.A. (2006)
    Serial detection of circulating tumour cells by reverse transcriptase-polymerase chain reaction assays is a marker for poor outcome in patients with malignant melanoma
    in BMC cancer (Online)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Palmieri G.; Satriano S.M.; Budroni M.; Cossu A.; Tanda F.; Canzanella S.; Caracò C.; Simeone E.; Daponte A.; Mozzillo N.; Comella G.; Castello G.; Ascierto P.A. (literal)
Pagina inizio
  • 266 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://www.biomedcentral.com/1471-2407/6/266 (literal)
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  • 6 (literal)
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  • article266pp8 (literal)
Note
  • Scopu (literal)
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Istituto di Chimica Biomolecolare-Consiglio Nazionale Ricerche, Li Punti-Sassari, Italy; Istituto Nazionale Tumori \"Fondazione Pascale\", Napoli, Italy; Centro Multizonale di Osservazione Epidemiologica, Azienda U.S.L. 1, Sassari, Italy; Servizio di Anatomia Patologica, Azienda U.S.L. 1, Sassari, Italy; 5 Associazione House Hospital Onlus, Napoli, Italy. (literal)
Titolo
  • Serial detection of circulating tumour cells by reverse transcriptase-polymerase chain reaction assays is a marker for poor outcome in patients with malignant melanoma (literal)
Abstract
  • Background: Detection of circulating malignant cells (CMCs) through a reverse transcriptase-polymerase chain reaction (RT-PCR) assay seems to be a demonstration of systemic disease. We here evaluated the prognostic role of RT-PCR assays in serially-taken peripheral blood samples from patients with malignant melanoma (MM). Methods: One hundred forty-nine melanoma patients with disease stage ranging from I to III were consecutively collected in 1997. A multi-marker RT-PCR assay was used on peripheral blood samples obtained at time of diagnosis and every 6 months during the first two years of follow-up (total: 5 samples). Univariate and multivariate analyses were performed after 83 months of median follow-up. Results: Detection of at least one circulating mRNA marker was considered a signal of the presence of CMC (referred to as PCR-positive assay). A significant correlation was found between the rate of recurrences and the increasing number of PCR-positive assays (P = 0.007). Presence of CMC in a high number (>=2) of analysed blood samples was significantly correlated with a poor clinical outcome (disease-free survival: P = 0.019; overall survival: P = 0.034). Multivariate analysis revealed that presence of a PCR-positive status does play a role as independent prognostic factors for overall survival in melanoma patients, adding precision to the predictive power of the disease stage. Conclusion: Our findings indicated that serial RT-PCR assay may identify a high risk subset of melanoma patients with occult cancer cells constantly detected in blood circulation. Prolonged presence of CMCs seems to act as a surrogate marker of disease progression or a sign of more aggressive disease. (literal)
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