Microsatellite instability and mutation analysis of candidate genes in unselected sardinian patients with endometrial carcinoma. (Articolo in rivista)

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  • Microsatellite instability and mutation analysis of candidate genes in unselected sardinian patients with endometrial carcinoma. (Articolo in rivista) (literal)
Anno
  • 2002-01-01T00:00:00+01:00 (literal)
Alternative label
  • Baldinu P, Cossu A, Manca A, Satta MP, Pisano M, Casula M, Dessole S, Pintus A, Tanda F, Palmieri G. (2002)
    Microsatellite instability and mutation analysis of candidate genes in unselected sardinian patients with endometrial carcinoma.
    in Cancer (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Baldinu P, Cossu A, Manca A, Satta MP, Pisano M, Casula M, Dessole S, Pintus A, Tanda F, Palmieri G. (literal)
Pagina inizio
  • 3157 (literal)
Pagina fine
  • 3168 (literal)
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  • 94 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
  • Su un gruppo di pazienti con carcinoma dell'endometrio che presentavano instabilità microsatellitare (MSI), è stato effettuato uno studio allo scopo di accertare la presenza sia del \"fenotipo mutatore\" (presenza di instabilità microsatellitare e espressione anomala di geni coinvolti nel mismatch repair o MMR) che di mutazioni germinali nei geni hMLH1, hMSH2 e PTEN. 25 tumori (34%) di pazienti con MSI risultano negativi alla colorazione immunoistochimica con anticorpi antihMLH1/hMSH2, mentre è stata trovata una sola nuova mutazione (1 paziente su 27) nei pazienti con fenotipo mutatore. Questi risultati suggeriscono il coinvolgimento di meccanismi epigenetici di inattivazione nella tumorigenesi endometriale. La mancanza di correlazione tra la sopravvivenza dei due gruppi di pazienti (MSI positivi e MSI negativi) non permette di attribuire valore prognostico all'instabilità microsatellitare. (literal)
Note
  • ISI Web of Science (WOS) (literal)
  • PubMed (literal)
  • Scopus (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Baldinu P., Casula.M., Colombino M., Manca A., Palmieri G., Pisano M., Satta M.P: Istituto di Chimica Biomolecolare, CNR, Sassari. Cossu A., Pintus A, Tanda F.: Istituto di Anatomia Patologica, Università di Sassari, Sassari. Dessole S.: Dipartimento di Ostetricia e Ginecologia, Università di Sassari, Sassari (literal)
Titolo
  • Microsatellite instability and mutation analysis of candidate genes in unselected sardinian patients with endometrial carcinoma. (literal)
Abstract
  • BACKGROUND: Microsatellite instability (MSI) is due mostly to a defective DNA mismatch repair (MMR). Inactivation of the two principal MMR genes, hMLH1 and hMSH2, and the PTEN tumor suppressor gene seems to be involved in endometrial tumorigenesis. In this study, Sardinian patients with endometrial carcinoma (EC) were analyzed to assess the prevalence of both the mutator phenotype (as defined by the presence of MSI and abnormal MMR gene expression at the somatic level) and the hMLH1, hMSH2, and PTEN germline mutations among patients with MSI positive EC. METHODS: Paraffin embedded tissue samples from 116 consecutive patients with EC were screened for MSI by polymerase chain reaction-based microsatellite analysis. Immunohistochemistry (IHC) with anti-hMLH1 and anti-hMSH2 antibodies was performed on MSI positive tumor tissue sections. Germline DNA was used for mutational screening by denaturing high-performance liquid chromatography analysis and automated sequencing. RESULTS: Thirty-nine patients with EC (34%) exhibited MSI; among them, 25 tumor samples (64%) showed negative immunostaining for hMLH1/hMSH2 proteins (referred to as IHC negative). No disease-causing mutation within the coding sequences of the hMLH1/hMSH2 and PTEN genes was found in patients with EC who had the mutator phenotype (MSI positive and IHC negative), except for a newly described hMLH1 missense mutation, Ile655Val, that was observed in 1 of 27 patients (4%). Although MSI was more common among patients with advanced-stage EC and increased as the tumor grade increased, no significant correlation with disease free survival or overall survival was observed among the two groups (MSI positive or MSI negative) of patients with EC. CONCLUSIONS: In patients with MSI positive EC, epigenetic inactivations rather than genetic mutations of the MMR genes seem to be involved in endometrial tumorigenesis. No prognostic value was demonstrated for MSI in patients with EC (literal)
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