http://www.cnr.it/ontology/cnr/individuo/prodotto/ID145198
Method for detecting toxic and non toxic cyanobacteria (Brevetto)
- Type
- Label
- Method for detecting toxic and non toxic cyanobacteria (Brevetto) (literal)
- Anno
- 2004-01-01T00:00:00+01:00 (literal)
- Alternative label
SIVONEN, K; RANTALA, A; Rouhianen, L; Fewer, D; Rajaniemi, P; Wilmotte, A; Boutte, C; Grubisic, S; Balthasart, P; De Bellis, G; Rizzi, E; Frosini, A; Castiglioni, B; Ventura, S; Mugnai, M (2004)
Method for detecting toxic and non toxic cyanobacteria
(literal)
- Titolo
- Method for detecting toxic and non toxic cyanobacteria (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- SIVONEN, K; RANTALA, A; Rouhianen, L; Fewer, D; Rajaniemi, P; Wilmotte, A; Boutte, C; Grubisic, S; Balthasart, P; De Bellis, G; Rizzi, E; Frosini, A; Castiglioni, B; Ventura, S; Mugnai, M (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
- Sequenze di oligonucleotidi per microarray specifiche per il riconoscimento di cianobatteri tossici e non tossici. (literal)
- Http://www.cnr.it/ontology/cnr/brevetti.owl#ricaduteEconomicheOccupazionali
- non prevedibili al momento (literal)
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- International Bureau (literal)
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- http://www.wipo.int/patentscope/search/en/WO2004104211 (literal)
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- Numero brevetto
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- Il brevetto è stato presentato dall'ISE in collaborazione con l'Università di Helsinki, l'Università di Liegi e ITB-CNR in accordo con la Società finlandese Seppo Laine. (literal)
- Anno di deposito
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- HELSINGIN YLIOPISTO [FI/FI]; Yliopistonkatu 4, PL 33, FI-00014 Helsingin Yliopisto (FI) (For All Designated States Except US).
UNIVERSITY OF LIEGE [BE/BE]; Quai van Beneden, 25, B-4020 Liege (BE) (For All Designated States Except US).
CONSIGLIO NAZIONALE DELLE RICERCHE [IT/IT]; Instituto di Technologie Biomediche, Via Fratelli Cervi, 93, I-20090 Segrate Milano (IT) (For All Designated States Except US).
CONSIGLIO NAZIONALE DELLE RICERCHE [IT/IT]; Instituto per lo Studio degli Ecosistemi, Largo Vittorio Tonolli, 50/52, I-28922 Pallanza Verbania (IT) (For All Designated States Except US). (literal)
- Titolo
- Method for detecting toxic and non toxic cyanobacteria (literal)
- Http://www.cnr.it/ontology/cnr/brevetti.owl#trasferimentoBrevetto
- Possibili utilizzatori del brevetto saranno industrie che producono kit per l'identificazione rapida di microrganismi in campioni naturali mediante estrazione di DNA (literal)
- Abstract
- METHOD FOR DETECTING TOXIC AND NON-TOXIC CYANOBACTERIA
This invention relates to a method for detecting toxic and non-toxic cyanobacteria. This invention relates also to oligonucleotides, which can be used in the detection method.
BACKGROUND OF THE INVENTION
Cyanobacteria produce a wide variety of bioactive compounds. Many of these are potent toxins, which cause health problems for animals and humans when producer organisms occur in masses in lakes and water reservoirs (Sivonen and Jones, 1999). Most well known of the cyanobacterial toxins are the hepatotoxic heptapeptides, microcystins. The general structure of microcystins is cyc o(-D-Ala-X-D-MeAsp-Z-Adda-D-Glu-Mdha-), where X and Z are variable L-amino acids, D-MeAsp is D-eryt zro-?-methylaspartic acid, Mdha is N-methyldehydroalanine and Adda is 3-an ino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid. More than 65 structurally different microcystins are known (Sivonen and Jones, 1999). Most common variants have L-leucine and L-arginine in the positions of X and Z, respectively, and demethylated forms are also frequently found. Toxicity of microcystins is caused by the inhibition of protein phosphatases 1 and 2A (MacKintosh et al., 1990). The level of inhibition varies depending on the structure, but the Adda and D-Glu moieties, which are almost invariable in microcystins, are essential for the inhibition (Goldberg et al., 1995) and hence for the toxicity. .
Microcystins have been found predominantly in cyanobacteria of three planktonic, bloom-forming genera, Anabaena, Microcystis and Planktothrix (Sivonen and Jones, 1999). Not all members of these genera make microcystins and both toxic and non-toxic strains occur in the same species. Toxic and non-toxic strains of Anabaena, Microcystis or Planktothrix cannot be separated based on the classical morphological taxonomy or ribosomal gene sequencing (Lyra et al., 2001). On the other hand, one strain may produce different microcystins and also other peptides simultaneously (Sivonen et al., 1992; Fujii et al., 1996; Fastner et al., 2001.
Peptide synthetase genes were shown to be required for the synthesis of microcystins
(Dittmann et al., 1997). Recently, the gene clusters encoding microcystin synthetase were sequenced and characterized from the unicellular Microcystis aeruginosa (Nishizawa et al., 2000; Tillet et al, 2000) and from the filamentous Planktothrix agardhii (Christiansen et al., 2003). It was demonstrated that the microcystins biosynthesis is a combination of peptide and polyketide synthesis (Nishizawa et al., 2000; Tillet et al., 2000).
The microcystin synthetase gene region spans about 55 kb, and includes genes for peptide synthetases (mcyA, -B, -C), polyketide synthases (mcyD), mixed peptide synthetase and polyketide synthases (mcyE, -G), and tailoring enzymes Tillett. et al. (2000),
Nishizawa etal (2000).
Microcystin producers among the filamentous, nitrogen-fixing genus, Anabaena, are found in North America, in France and in Northern Europe, where they frequently develop massive growth in lakes and reservoirs (Sivonen and Jones, 1999). The bioactive peptides produced by Anabaena 90 have been characterized: three microcystins (MCYST-LR, MCYST-RR and D-Asp-MCYST-LR; Sivonen et al., 1992), two seven-residue depsipeptides (anabaenopeptilide 90 A and 90B), and three six-residue peptides having an ureido linkage (anabaenopeptins A, B and C; Fujii et al., 1996). However, the microcystin synthetase gene region from Anabaena has not been sequenced.
Based on the sequence data available, various DNA probes and primers have been designed and used to discriminate between toxic microcystin-producing and non-toxic non-microcystin producing genotypes by hybridization and PCR. However, the existing primers deduced from Microcystis mcy genes, reliably identify potential microcystin-producers only in Microcystis and fail to amplify mcy sequences from part of microcystin containing strains of other genera. There is therefore a great need for oligonucleotides, which could be used as probes and primers in detecting toxic cyanobacteria also in genera other than Microcystis. Such oligonucleotides should discriminate between toxic microcystin-producing and non-toxic non-microcystin producing genotypes in various molecular biology methods, such oligonucleotides should be specific to the studied cyanobacteria genera and the oligonucleotides should be able to discriminate the most important or dominating microcystin producing cyanobacteria genera from one another.
It would be also of advantage if non-toxic cyanobacteria could be identified. (literal)
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