http://www.cnr.it/ontology/cnr/individuo/prodotto/ID144731

Sus scrofa natriuretic peptide receptor 2 mRNA, partial cds (Brevetto)

Type

Brevetto (Classe)
Brevetto (Classe)
Prodotto della ricerca (Classe)

Label

Sus scrofa natriuretic peptide receptor 2 mRNA, partial cds (Brevetto) (literal)

Anno

2006-01-01T00:00:00+01:00 (literal)

Alternative label

Del Ry S.; Cabiati M.; Giannessi D. (2006)
Sus scrofa natriuretic peptide receptor 2 mRNA, partial cds
(literal)

Titolo

Sus scrofa natriuretic peptide receptor 2 mRNA, partial cds (literal)

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Del Ry S.; Cabiati M.; Giannessi D. (literal)

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NIH (literal)

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Internazionale (literal)

Numero brevetto

DQ487044 (literal)

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Patent n. DQ487044, GENE BANK - USA. Registered on 2006-4-10. (literal)

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Background: Recent studies have demonstrated that C-type natriuretic peptide (CNP), a member of the family of natriuretic peptides, is found not only in the heart, but that it is also elevated during heart failure (HF) where it may elicit important compensatory physiological consequences on ventricular remodeling. The cardiovascular actions of CNP are mediated mainly via interaction with natriuretic peptide receptor-B (NPR-B), whose pathophysiological role on the cardiovascular function is not yet completely determined. Aim: In the context of a wide study devoted to assess the possible role and function of CNP and its main receptor NPR-B, in a pig experimental model of pacing induced HF, we sequenced and determined the cardiac expression of NPR-B in Sus Scrofa, lacking in GenBank, and evaluated its homology with murine and human species. Method: Total RNA was extracted from samples obtained from mouse heart and from atrium and ventricle of normal and HF pig by guanidinium thyocyanate-phenol-chloroform method. Polymerase chain reaction primers designed from consensus sequences of mouse were used to sequence the pig NPR-B gene and the bands of interest were excised from the gel and processed with a Gel extraction kit protocol. The purified DNA was electrophoresed on agarose gels together with a known concentation DNA marker and the bands were analyzed by Quantity One Software. The DNA was sequenced with Sanger method using Applayed Biosystems ABI PRISM 3730 XL DNA Sequencer (BM (literal)

Anno di deposito