Surface immobilization of fibronectin-derived PHSRN peptide on functionalized polymer film - Effects on fibroblast spreading (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Surface immobilization of fibronectin-derived PHSRN peptide on functionalized polymer film - Effects on fibroblast spreading (Articolo in rivista) (literal)

Anno

• 2010-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.jcis.2009.09.046 (literal)

Alternative label

• C. Satriano; G.M.L. Messina; C. Marino; I. Aiello; E. Conte; D. La Mendola; D. Distefano; F. D'Alessandro; G. Pappalardo; G. Impellizzeri. (2010)
  Surface immobilization of fibronectin-derived PHSRN peptide on functionalized polymer film - Effects on fibroblast spreading
  in Journal of colloid and interface science (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• C. Satriano; G.M.L. Messina; C. Marino; I. Aiello; E. Conte; D. La Mendola; D. Distefano; F. D'Alessandro; G. Pappalardo; G. Impellizzeri. (literal)

Pagina inizio

• 232 (literal)

Pagina fine

• 239 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 341 (literal)

Rivista

• Journal of colloid and interface science (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Cristina Satriano, Department of Chemical Sciences, Catania University, Italy Grazia M.L. Messina, Department of Chemical Sciences, Catania University, Italy Clara Marino, Department of Chemical Sciences, Catania University, Italy Ivana Aiello, Department of Chemical Sciences, Catania University, Italy Enrico Conte, Department of Chemical Sciences, Catania University, Italy Donatella A. Distefano, Department of Chemical Sciences, Catania University, Italy Franca D'Alessandro, Department of Chemical Sciences, Catania University, Italy Giuseppe Impellizzeri, Department of Chemical Sciences, Catania University, Italy (literal)

Titolo

• Surface immobilization of fibronectin-derived PHSRN peptide on functionalized polymer film - Effects on fibroblast spreading (literal)

Abstract

• The Pro-His-Ser-Arg-Asn (PHSRN) sequence in fibronectin is a second cell-binding site that synergistically affects Arg-Gly-Asp (RGD). The PHSRN peptide also induces cell invasion and accelerates wound healing. We report on the surface immobilization of PHSRN by spontaneous adsorption on polysiloxane thin films which have different surface free energy characteristics. Low-surface energy (hydrophobic) polysiloxane and the corresponding high-surface energy (hydrophilic) surfaces obtained by UV-ozone treatments were used as adsorbing substrates. The peptide adsorption process was investigated by quartz crystal microbalance with dissipation monitoring and atomic force microscopy. Both adsorption kinetics and peptide rearrangement dynamics at the solid interface were significantly different on the surface-modified films compared to the untreated ones. Fibroblast cells cultures at short times and in a simplified environment, i.e., a medium-free solution, were prepared to distinguish interaction events at the interface between cell membrane and surface-immobilized peptide for the two cases. It turned out that the cell-adhesive effect of immobilized PHSRN was different for hydrophobic compared to hydrophilic ones. Early signatures of cell spreading were only observed on the hydrophilic substrates. These effects are explained in terms of different spatial arrangements of PHSRN molecules immobilized on the two types of surfaces. (literal)

Prodotto di

• Progettazione e sintesi di nuovi composti bioattivi e loro veicolazione sito specifica. (PM.P01.003.002) (Modulo)
• Institute of biostructure and bioimaging (IBB) (Istituto)

Autore CNR

• GIUSEPPE PAPPALARDO (Unità di personale interno)
• DIEGO LA MENDOLA (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "Surface immobilization of fibronectin-derived PHSRN peptide on functionalized polymer film - Effects on fibroblast spreading" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• GIUSEPPE PAPPALARDO (Unità di personale interno)
• DIEGO LA MENDOLA (Unità di personale interno)

Prodotto

• Institute of biostructure and bioimaging (IBB) (Istituto)
• Progettazione e sintesi di nuovi composti bioattivi e loro veicolazione sito specifica. (PM.P01.003.002) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of colloid and interface science (Rivista)

Insieme di parole chiave di

• Parole chiave di "Surface immobilization of fibronectin-derived PHSRN peptide on functionalized polymer film - Effects on fibroblast spreading" (Insieme di parole chiave)