VEGFR1D2 in drug discovery: Expression and molecular characterization (Articolo in rivista)

Type
Label
  • VEGFR1D2 in drug discovery: Expression and molecular characterization (Articolo in rivista) (literal)
Anno
  • 2010-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1002/bip.21448 (literal)
Alternative label
  • Di Stasi R, Diana D, Capasso D, Palumbo R, Romanelli A, Pedone C, Fattorusso R, D'Andrea LD (2010)
    VEGFR1D2 in drug discovery: Expression and molecular characterization
    in Biopolymers (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Di Stasi R, Diana D, Capasso D, Palumbo R, Romanelli A, Pedone C, Fattorusso R, D'Andrea LD (literal)
Pagina inizio
  • 800 (literal)
Pagina fine
  • 809 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 94 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Di Stasi R, CNR, CNR, Ist Biostrutture & Bioimmagini, I-80134 Naples, Italy Diana D, CNR, CNR, Ist Biostrutture & Bioimmagini, I-80134 Naples, Italy Capasso D, Univ Naples Federico 2, Dipartimento Sci Biol, Naples, Italy Palumbo R, CNR, CNR, Ist Biostrutture & Bioimmagini, I-80134 Naples, Italy Romanelli A, Univ Naples Federico 2, Dipartimento Sci Biol, Naples, Italy Pedone C, Univ Naples Federico 2, Dipartimento Sci Biol, Naples, Italy Fattorusso R, Seconda Univ Napoli, Dipartimento Sci Ambientali, Caserta, Italy D'Andrea LD, CNR, CNR, Ist Biostrutture & Bioimmagini, I-80134 Naples, Italy (literal)
Titolo
  • VEGFR1D2 in drug discovery: Expression and molecular characterization (literal)
Abstract
  • Vascular endothelial growth factor (VEGF) is a potent angiogenic factor Its biological activity is mediated by the binding to the extracellular domain of two tyrosine kinase transmembrane receptors VEGFR1 and VEGFR2 Deletion studies showed that VEGF binding site resides in the first three domains of VEGFR1 and in domains 2 and 3 of VEGFR2 In particular, the second extracellular domain of VEGFR1 (VEGFR1(D2)) contains most of the VEGF binding requirements Here, we report an efficient expression protocol and the molecular characterization by spectroscopic techniques of VEGFR1D2 The protein was expressed in E cob and refolded from inclusion bodies The recombinant protein assumes the correct fold as assessed by a combination of biochemical and functional assays as well as by NMR characterization Furthermore, the recombinant VEGFR1(D2) was analyzed by circular dichroism and fluorescence spectroscopy The protein obtained by this procedure is suitable for the structural characterization of the complexes with receptor binders and to be used in interaction/screening studies (literal)
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