http://www.cnr.it/ontology/cnr/individuo/prodotto/ID14139
Copper binds the TFF1 carboxy-terminal tail favoring its homodimerization and motogenic activity (Articolo in rivista)
- Type
- Label
- Copper binds the TFF1 carboxy-terminal tail favoring its homodimerization and motogenic activity (Articolo in rivista) (literal)
- Anno
- 2010-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1007/s00018-010-0309-7 (literal)
- Alternative label
Tosco A, Monti MC, Fontanella B, Montefusco S, D'Andrea LD, Ziaco B, Rio MC, Marzullo L. (2010)
Copper binds the TFF1 carboxy-terminal tail favoring its homodimerization and motogenic activity
in Cellular and molecular life sciences (Print. ed.)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Tosco A, Monti MC, Fontanella B, Montefusco S, D'Andrea LD, Ziaco B, Rio MC, Marzullo L. (literal)
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- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Tosco A, Monti MC, Fontanella B, Montefusco S,Rio MC, Marzullo L., Università di Salerno
D'Andrea LD, Ziaco B; Istituto di Biostrutture e Bioimmagini, CNR (literal)
- Titolo
- Copper binds the TFF1 carboxy-terminal tail favoring its homodimerization and motogenic activity (literal)
- Abstract
- Trefoil protein 1 (TFF1) is a small secreted protein belonging to the trefoil factor family of proteins, that are present mainly in the gastrointestinal (GI) tract and play pivotal roles as motogenic factors in epithelial restitution, cell motility, and other incompletely characterized biological processes. We previously reported the up-regulation of TFF1 gene in copper deficient rats and the unexpected property of the peptide to selectively bind copper. Following the previous evidence, here we report the characterization of the copper binding site by fluorescence quenching spectroscopy and mass spectrometric analyses. We demonstrate that Cys58 and at least three Glu surrounding residues surrounding it, are essential to efficiently bind copper. Moreover, copper binding promotes the TFF1 homodimerization, thus increasing its motogenic activity in in vitro wound healing assays. Copper levels could then modulate the TFF1 functions in the GI tract, as well as its postulated role in cancer progression and invasion. (literal)
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