Enhancement of reaction conditions for the radiolabelling of DOTA-peptides with high activities of yttrium-90 (Articolo in rivista)

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Label
  • Enhancement of reaction conditions for the radiolabelling of DOTA-peptides with high activities of yttrium-90 (Articolo in rivista) (literal)
Anno
  • 2011-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.apradiso.2010.09.001 (literal)
Alternative label
  • Nardelli, A (1); Castaldi, E (1); Ortosecco, G (1); Speranza, A (1); Storto, G (2); Pace, L (3); Salvatore, M (3). (2011)
    Enhancement of reaction conditions for the radiolabelling of DOTA-peptides with high activities of yttrium-90
    in Applied radiation and isotopes; Pergamon-Elsevier Press Science LDT, Oxford (Regno Unito)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Nardelli, A (1); Castaldi, E (1); Ortosecco, G (1); Speranza, A (1); Storto, G (2); Pace, L (3); Salvatore, M (3). (literal)
Pagina inizio
  • 52 (literal)
Pagina fine
  • 55 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://www.sciencedirect.com/science/article/pii/S0969804310003623 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 69 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1) Istituto di Biostrutture e Bioimmagini, Consiglio Nazionale delle Ricerche (CNR), Naples, Italy. 2) IRCCS-CROB, Rionero in Vulture, Italy. 3) Dipartimento di Scienze Biomorfologiche e Funzionali, Universit?a ''FedericoII'', ViaPansini 5, 80131 Naples, Italy. (literal)
Titolo
  • Enhancement of reaction conditions for the radiolabelling of DOTA-peptides with high activities of yttrium-90 (literal)
Abstract
  • The achievement of high specific activities during procedures has been indicated as the critical factor to consent effective therapy Several radiochemical factors may negatively impact reaction procedures such as pH temperature and time of reaction Our study was undertaken to explore the influence of radiochemical parameters such as time of incubation on reaction kinetics during the radiolabelling of DOTATATE with (90)Y Methods Forty-five radiolabelling procedures were carried out using small volumes of yttrium-90 typically 60-78 mu L. At nearly constant pH and temperature two different settings of radiolabelling procedures were implemented removing the products from the heating water bath approximately after 30 min (group E early n=20) and after 39 min (group L, later n=25) Quality controls were performed by means of both high-performance liquid chromatography (HPLC) and thin-layer chromatography (TLC) Results Reaction kinetics for (90)Y were found to a provide suitable percentage of incorporation at pH 4 5 for both groups Reaction temperature was not different between groups E and L A significant difference was found between the two groups in radiochemical yield which was 95 6% +/- 0 8 for group E and 98 2% +/- 11 for group L (p < 0 0001) The specific activity of the final product was 46 9 MBq/nmol Conclusion In order to achieve optimal specific activities pH temperature and time of reaction necessitate careful evaluation and setting A statistically significant difference in labelling yield was found between a set of procedures completed at 39 min as compared to that executed at 30 min keep the reaction pH and temperature constant. (literal)
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